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Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, September 7, 2010. Thank you for your patience.

Cholesterol Assay Kit

Cayman Chemical Item Number 10007640

Cholesterol Assay Kit

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Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Description

Cholesterol, particularly in the form of low density lipoproteins (LDL), is well understood to be associated with increased risk of coronary heart disease. The measurement of cholesterol is one of the most common tests performed in the clinical laboratory setting. However, simple and easy assays for cholesterol in the research lab have not been readily available. The Cayman’s Cholesterol Assay provides a simple fluorometric method for the sensitive quantitation of total cholesterol in plasma or serum. In our assay, cholesteryl esters in the sample are first hydrolyzed by cholesterol esterase. All cholesterol, whether previously esterified or existing free in the circulation, is then oxidized by cholesterol oxidase to the corresponding ketone and hydrogen peroxide. ADHP (10-acetyl-3,7-dihydroxyphenoxazine) is utilized as a highly sensitive and stable probe for hydrogen peroxide. Horseradish peroxidase catalyzes the reaction of ADHP with hydrogen peroxide to yield the highly fluorescent product resorufin which can be monitored using excitation wavelengths of 530-580 nm and emission wavelengths of 585-595 nm.

Related Articles

Matsuura, E., Lopez, L.R. Are oxidized LDL/β2-glycoprotein I complexes pathogenic antigens in autoimmune-mediated atherosclerosis?. Clin Dev Immunol 11(2) 103-111 (2004).

Linsel-Nitschke, P., Tall, A.R. HDL as a target in the treatment of atherosclerotic cardiovascular disease. Nat Rev Drug Discov 4 193-205 (2005).

Kishi, K., Ochiai, K., Ohta, Y., et al. Highly sensitive cholesterol assay with enzymatic cycling applied to measurement of remnant lipoprotein-cholesterol in serum. Clin Chem 48(5) 737-741 (2002).

Li, D., Sinclair, A., Mann, N., et al. The association of diet and thrombotic risk factors in healthy male vegetarians and meat-eaters. Eur J Clin Nutr 53 612-619 (1999).

Jauhiainen, M., Dolphin, P.J. Human plasma lecithin-cholesterol acyltransferase. An elucidation of the catalytic mechanism. J Biol Chem 261(15) 7032-7043 (1986).

Ledwozyw, A., Michalak, J., Stepien, A., et al. The relationship between plasma triglycerides, cholesterol, total lipids, and lipid peroxidation products during human atherosclerosis. Clin Chim Acta 155 275-284 (1986).

McNamara, D.J., Kolb, R., Parker, T.S., et al. Heterogeneity of cholesterol homeostasis in man. Response to changes in dietary fat quantity and cholesterol quanity. J Clin Invest 79 1729-1739 (1987).

Amundson, D.M., Zhou, M. Fluorometric method for the enzymatic determination of cholesterol. J Biochem Biophys Meth 38 43-52 (1999).

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10007640-1EA
Cholesterol Assay Buffer (10X) 1 ea
Cholesterol Assay Standard 1 ea
Cholesterol Assay Detector 2 × 1 ea
Cholesterol Assay Horseradish Peroxidase 1 ea
Cholesterol Assay Oxidase 1 ea
Cholesterol Assay Esterase 1 ea
Cholesterol Assay Dimethyl Sulfoxide 1 ea
96-Well Plate (black)
10007640-5EA
Cholesterol Assay Buffer (10X) 5 ea
Cholesterol Assay Standard 5 ea
Cholesterol Assay Detector 5 × 1 ea
Cholesterol Assay Horseradish Peroxidase 5 ea
Cholesterol Assay Oxidase 5 ea
Cholesterol Assay Esterase 5 ea
Cholesterol Assay Dimethyl Sulfoxide 5 ea
96-Well Plate (black)

Pricing updated 2010-09-02. Prices are subject to change without notice.

Pricing is for North America only. Other customers should contact a distributor in their region.

Cayman strives to be a reliable biochemical reagent vendor by providing the best possible products and services. To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Cholesterol Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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