Antigen: GPR35 amino acids 295-304 (AKAHKSQDSL) · Host: rabbit · Application(s): WB and IHC (paraffin embedded sections); other applications not tested · Many G protein-coupled receptor 35 (GPR35) ligands have recently been identified including kynurenic acid and zaprinast.^1,2 Multiple isoforms of this receptor have been reported and GPR35 mRNAs were detected in several tissues including intestine, lymphocytes, skeletal muscle, pancreatic β cells, and tumor cell lines.^1,3 Cayman’s GPR35 polyclonal antibody has been used successfully to detect this receptor in human and mouse intestine samples as well as in human, mouse, and porcine lymphocytes at 30 kDa on immunoblot.

1 Wang, J., Simonavicius, N., Wu, X., et al. Kynurenic acid as a ligand for orphan G protein-coupled receptor GPR35. J Biol Chem 281(31) 22021-22028 (2006).

2 Taniguchi, Y., Tonai-Kachi, H., and Shinjo, K. Zaprinast, a well-known cyclic guanosine monophosphate-specific phosphodiesterase inhibitor, is an agonist for GPR35. FEBS Lett 580 5003-5008 (2006).

3 Okumura, S., Baba, H., Kumada, T., et al. Cloning of a G-protein-coupled receptor that shoes an activity to transform NIH3T3 cells and is expressed in gastric cancer cells. Cancer Sci 95(2) 131-135 (2004).

Okumura, S., Baba, H., Kumada, T., et al. Cloning of a G-protein-coupled receptor that shoes an activity to transform NIH3T3 cells and is expressed in gastric cancer cells. Cancer Sci 95(2) 131-135 (2004).

Wang, J., Simonavicius, N., Wu, X., et al. Kynurenic acid as a ligand for orphan G protein-coupled receptor GPR35. J Biol Chem 281(31) 22021-22028 (2006).

Taniguchi, Y., Tonai-Kachi, H., and Shinjo, K. Zaprinast, a well-known cyclic guanosine monophosphate-specific phosphodiesterase inhibitor, is an agonist for GPR35. FEBS Lett 580 5003-5008 (2006).