Antigen: Synthetic peptide from mouse LDL receptor amino acids 499-511 (SVADTKGVKRRTL) · Host: rabbit · Application(s): WB and ICC; other applications not tested · Cholesterol is an essential element of cell membranes and is carried around the body packaged in lipoproteins, primarily low-density lipoproteins (LDLs). The LDL receptors (LDLRs) are cell surface glycoproteins that scavenge LDL from the blood and regulate plasma LDL cholesterol. LDLRs contain five primary domains: the ligand binding domain, the homology with the EGF precursor domain, the O-linked sugars domain, the membrane-spanning region, and a cytoplasmic tail.¹ In humans, more than 60% of the LDL R3 are found in the liver.² LDLR expression is under hormonal control both in vivo and in vitro. Mutations in the LDLR gene cause disorders such as familial hypercholesterolemia and atherosclerosis.¹ Murine LDLR is 864 amino acids in length with an estimated molecular weight of 95 kDa. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.¹ Cayman’s LDL receptor polyclonal antibody detects both glycosylated and unglycosylated proteins in tissue/cell samples such as liver, Hep-G₂, and RAW 264.7 cells.

1 Goldstein, J.L., Brown, M.S., Anderson, R.G.W., et al. Receptor-mediated endocytosis: Concepts emerging from the LDL receptor system. Annu Rev Cell Biol 1 1-39 (1985).

2 Rudling, M.J., Reihnér, E., Einarsson, K., et al. Low density lipoprotein receptor-binding activity in human tissues: Quantitative importance of hepatic receptors and evidence for regulation of their expression in vivo. Proc Natl Acad Sci USA 87 3469-3473 (1990).

Goldstein, J.L., Brown, M.S., Anderson, R.G.W., et al. Receptor-mediated endocytosis: Concepts emerging from the LDL receptor system. Annu Rev Cell Biol 1 1-39 (1985).

Rudling, M.J., Reihnér, E., Einarsson, K., et al. Low density lipoprotein receptor-binding activity in human tissues: Quantitative importance of hepatic receptors and evidence for regulation of their expression in vivo. Proc Natl Acad Sci USA 87 3469-3473 (1990).