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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Prostaglandin D Synthase (lipocalin-type; human) EIA Kit Exclusive

Cayman Chemical Item Number 10007684

Lipocalin-PGDS ELISA Kit; L-PGDS EIA Kit

Prostaglandin D Synthase (lipocalin-type; human) EIA Kit

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Prostaglandin D Synthase (lipocalin-type; human) EIA Kit Analysis Tools

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Description

Limit of detection: 6 ng/ml · Prostaglandin D synthase (PGDS) catalyzes the isomerization of PGH2 to produce PGD2, a potent lipid mediator involved in sleep regulation, nociception, platelet aggregation, and allergic and inflammatory responses. Lipocalin-type PGDS (a.k.a. β-trace) is a homodimer with each subunit ranging in size from 20-31 kDa, depending on the extent of glycosylation. L-PGDS has two functions: it catalyzes the conversion of PGH2 to PGD2 and acts as a carrier protein for lipid-like molecules (i.e., retinoids and thyroid hormones).1 L-PGDS is present in a variety of body fluids including cerebrospinal fluid, seminal fluid, and plasma. Cayman’s L-PGDS (human) EIA is an immunometric (i.e., sandwich) EIA with a standard curve ranging from 0-100 ng/ml and a limit of detection of 6 ng/ml. Inter- and intra-assay CV’s of less than 15% may be achieved at most concentrations. This assay has been validated using cerebrospinal fluid which contains approximately 12-30 µg/ml of L-PGDS.

1 Urade, Y., and Hayaishi, O. Biochemical, structural, genetic, physiological, and pathophysiological features of lipocalin-type prostaglandin D synthase. Biochim Biophys Acta 1482 259-271 (2000).

Synonyms
  • Lipocalin-PGDS ELISA Kit
  • L-PGDS EIA Kit
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
Prostaglandin D Synthase (lipocalin-type; human) 100%

Background Reading

Urade, Y., Watanabe, K., and Hayaishi, O. Prostaglandin D, E, and F synthases. J Lipid Mediat Cell Signal 12 257-273 (1995).

Kanaoka, Y., Fujimori, K., Kikuno, R., et al. Structure and chromosomal localization of human and mouse genes for hematopoietic prostaglandin D synthase. Eur J Biochem 267 3315-3322 (2000).

Kanaoka, Y., Ago, H., Inagaki, E., et al. Cloning and crystal structure of hematopoietic prostaglandin D synthase. Cell 90 1085-1095 (1997).

Urade, Y., Fujimoto, N., Ujihara, M., et al. Biochemical and immunological characterization of rat spleen prostaglandin D synthetase. J Biol Chem 262(8) 3820-3825 (1987).

Jowsey, I.R., Thomson, A.M., Flanagan, J.U., et al. Mammalian class sigma glutathione s-transferases: Catalytic properties and tissue-specific expression of human and rat GSH-dependent prostaglandin D2 synthases. Biochem J 359 507-516 (2001).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

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10007684-96well
Anti-L-PGDS (human) EIA Strip Plate 1 ea
L-PGDS (human) Biotinylated Detection Antibody 100 dtn
L-PGDS (human) EIA Standard 3 × 1 ea
Polysorbate 20 1 ea
Avidin-Acetylcholinesterase Conjugate 10 mL
Ellman's Reagent 3 × 100 dtn
EIA Buffer Concentrate (10X) 2 × 10 mL
Wash Buffer Concentrate (400X) 5 mL
Non-specific Mouse Serum 100 dtn
10007684-480well
Anti-L-PGDS (human) EIA Strip Plate 5 ea
L-PGDS (human) Biotinylated Detection Antibody 500 dtn
L-PGDS (human) EIA Standard 6 × 1 ea
Polysorbate 20 1 ea
Avidin-Acetylcholinesterase Conjugate 50 mL
Ellman's Reagent 6 × 250 dtn
EIA Buffer Concentrate (10X) 4 × 10 mL
Wash Buffer Concentrate (400X) 12.5 mL
Non-specific Mouse Serum 500 dtn
Size Price Quantity Subtotal
96 wells $330.00 $0.00
480 wells $1,238.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Prostaglandin D Synthase (lipocalin-type; human) EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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