See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Leukotriene A4 Hydrolase (human recombinant)

Cayman Chemical Item Number 10007817

LTA4H

See more

Description

Source: recombinant C-terminal hexahistidine-tagged enzyme expressed in E. coli · Mr: ~69 kDa · Leukotriene A4 hydrolase (LTA4H) is a bifunctional zinc metalloenzyme that converts LTA4 into LTB4, a potent neutrophil chemoattractant.1 In addition to the hydrolase activity, LTA4H also exhibits anion-dependent aminopeptidase activity.1,2 LTA4H is a potential new drug target for a variety of indications associated with leukocyte infiltration to sites of inflammation. Cayman’s LTA4H (human recombinant) has been expressed and purified from E. coli. Both the epoxide hydrolase and aminopeptidase activities of the enzyme are functional as assessed by measurement of the separate enzyme activities. The enzyme therefore has application for the screening of inhibitors of LTB4 synthesis.

1 Haeggström, J.Z. Leukotriene A4 hydrolase/aminopeptidase, the gatekeeper of chemotactic leukotriene B4 biosynthesis. J Biol Chem 279(49) 50639-50642 (2004).

2 Rudberg, P.C., Tholander, F., Andberg, M., et al. Leukotriene A4 hydrolase: Identification of a common carboxylate recognition site for the epoxide hydrolase and aminopeptidase substrates. J Biol Chem 279(26) 27376-27382 (2004).

Synonyms
  • LTA4H
Formulation A solution in 100 mM Tris, pH 8.0, containing 100 mM potassium chloride and 20% glycerol
Purity >90%
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Haeggström, J.Z. Leukotriene A4 hydrolase/aminopeptidase, the gatekeeper of chemotactic leukotriene B4 biosynthesis. J Biol Chem 279(49) 50639-50642 (2004).

Funk, C.D., Rådmark, O., Fu, J.Y., et al. Molecular cloning and amino acid sequence of leukotriene A4 hydrolase. Proc Natl Acad Sci USA 84 6677-6681 (1987).

Minami, M., Ohno, S., Kawasaki, H., et al. Molecular cloning of a cDNA coding for human leukotriene A4 hydrolase. Complete primary structure of an enzyme involved in eicosanoid synthesis. J Biol Chem 262 13873-13876 (1987).

Mancini, J.A., and Evans, J.F. Coupling of recombinant 5-lipoxygenase and leukotriene A4 hydrolase activities and transcellular metabolism of leukotriene A4 in Sf9 cells. Eur J Biochem 218 477-484 (1993).

Samuelsson, B., and Funk, C.D. Enzymes involved in the biosynthesis of leukotriene B4. J Biol Chem 264 19469-19472 (1989).

Rudberg, P.C., Tholander, F., Andberg, M., et al. Leukotriene A4 hydrolase: Identification of a common carboxylate recognition site for the epoxide hydrolase and aminopeptidase substrates. J Biol Chem 279(26) 27376-27382 (2004).

Show all 6 Hide all but first 3
Size Price Quantity Subtotal
25 µg $115.00 $0.00
50 µg $219.00 $0.00
100 µg $414.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

Related Products

Leukotriene A4 Hydrolase Polyclonal Antibody
Leukotriene B4 EIA Kit
N-acetyl-Pro-Gly-Pro Peptide New
Prolyl-glycyl-proline Peptide New
Show all 4 Hide all but first 3

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?