SREBP-2 Transcription Factor Assay Kit

Cayman Chemical Item Number 10007819

Sterol Regulatory Element-binding Protein-2 Assay Kit

Description

Lipid homeostasis in vertebrate cells is regulated by a family of transcription factors called sterol regulatory element-binding proteins (SREBP’s). SREBP’s directly activate the expression of over 30 genes involved in the synthesis and uptake of cholesterol, fatty acids, triglycerides, and phospholipids.¹ Three different SREBP isoforms designated SREBP-1a, SREBP-1c, and SREBP-2 are encoded by two different genes and belong to the basic helix-loop-helix-leucine zipper (bHLH-ZIP) family of transcription factors.² SREBP-2 performs a critical role in the transcriptional regulation of genes involved in cholesterol synthesis and uptake including HMG-CoA synthase, HMG-CoA reductase, and the LDL receptor. Cayman’s SREBP-2 Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96 well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized to the wells of a 96 well plate. SREBP contained in a nuclear extract, binds specifically to the SREBP response element. SREBP is detected by addition of specific primary antibody directed against SREBP. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.

1 Lin, J., Yang, R., Tarr, P.T., et al. Hyperlipidemic effects of dietary saturated fats mediated through PGC-1β coactivation of SREBP. Cell 120 261-273 (2005).

2 Osborne, T.F. Sterol regulatory element-binding proteins (SREBP’s): Key regulators of nutritional homeostasis and insulin action. J Biol Chem 275(42) 32379-32382 (2000).

Synonyms

Sterol Regulatory Element-binding Protein-2 Assay Kit

Stability

6 months

Storage

-80°C

Shipping

Dry ice in continental US; may vary elsewhere

Background Reading

Lin, J., Yang, R., Tarr, P.T., et al. Hyperlipidemic effects of dietary saturated fats mediated through PGC-1β coactivation of SREBP. Cell 120 261-273 (2005).

Osborne, T.F. Sterol regulatory element-binding proteins (SREBP’s): Key regulators of nutritional homeostasis and insulin action. J Biol Chem 275(42) 32379-32382 (2000).

Brown, M.S., and Goldstein, J.L. The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89 331-340 (1997).

10007819-96WELL

Transcription Factor Binding Assay Buffer (4X)

1 ea

Transcription Factor Antibody Binding Buffer (10X)

1 ea

Transcription Factor Goat Anti-Rabbit HRP Conjugate

1 ea

Transcription Factor Developing Solution

1 ea

Transcription Factor Stop Solution

1 ea

Transcription Factor Reagent A