Antigen: phosphopeptide corresponding to amino acid residues surrounding phospho-Ser⁵²³ of human 5-lipoxygenase (5-LO) · Host: rabbit · Application(s): WB · The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs).¹ Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases such as asthma, fibrosis,² and atherosclerosis. Recent work has demonstrated that the activity of 5-LO is regulated by protein kinase A (PKA) phosphorylation of serine-523 in 5-LO. Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, may therefore contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser⁵²³ on 5-LO provides a valuable tool for studying the role of 5-LO regulation in diseases such as asthma, fibrosis, and atherosclerosis.

1 Funk, C.D. Prostaglandins and leukotrienes: Advances in eicosanoid biology. Science 294 1871-1875 (2001).

2 Wilborn, J., Bailie, M., Coffey, M., et al. Constitutive activation of 5-lipoxygenase in the lungs of patients with idiopathic pulmonary fibrosis. J Clin Invest 97 1827-1836 (1996).

Funk, C.D. Prostaglandins and leukotrienes: Advances in eicosanoid biology. Science 294 1871-1875 (2001).

Wilborn, J., Bailie, M., Coffey, M., et al. Constitutive activation of 5-lipoxygenase in the lungs of patients with idiopathic pulmonary fibrosis. J Clin Invest 97 1827-1836 (1996).

Luo, M., Jones, S.M., Phare, S.M., et al. Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on serine 523. J Biol Chem 279(40) 41512-41520 (2004).

Dwyer, J.H., Allayee, H., Dwyer, K.M., et al. Arachidonate 5-lipoxygenase promoter genotype, dietary arachidonic acid, and atherosclerosis. N Engl J Med 350(1) 29-37 (2004).

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