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Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

NF-κB (p65) Transcription Factor Assay Kit

Cayman Chemical Item Number 10007889

NF-κB (p65) Transcription Factor Assay Kit

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Description

Cayman’s NF-κB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-κB response element is immobilized to the wells of a 96 well plate. NF-κB contained in a nuclear extract, binds specifically to the NF-κB response element. NF-κB (p65) is detected by addition of specific primary antibody directed against NF-κB (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Cayman’s NF-κB (p65) Transcription Factor Assay detects human NF-κB (p65). It will not cross-react with NF-κB (p50).
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Gilmore, T.D. The Rel/NF-κB signal transduction pathway: Introduction. Oncogene 18 6842-6844 (1999).

Maeda, S., Hsu, L., Liu, H., et al. Nod2 mutation in Crohn’s disease potentiates NF-κB activity and IL-1β processing. Science 307 734-738 (2005).

Arkan, M.C., Hevener, A.L., Greten, F.R., et al. IKK-β links inflammation to obesity-induced insulin resistance. Nat Med 11(2) 191-198 (2005).

Gilroy, D.W., Lawrence, T., Perretti, M., et al. Inflammatory resolution: New opportunities for drug discovery. Nat Rev Drug Discov 3 401-416 (2004).

Pahl, H.L. Activators and target genes of Rel/NF-κB transcription factors. Oncogene 18 6853-6866 (1999).

Karin, M. The beginning of the end: IκB kinase (IKK) and NF-κB activation. J Biol Chem 274(39) 27339-27342 (1999).

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10007889-96WELL
Transcription Factor Binding Assay Buffer (4X) 1 ea
Transcription Factor Antibody Binding Buffer (10X) 1 ea
Transcription Factor Goat Anti-Rabbit HRP Conjugate 1 ea
Transcription Factor Developing Solution 1 ea
Transcription Factor Stop Solution 1 ea
Transcription Factor Reagent A 1 ea
Transcription Factor NF-κB 96-Well Strip Plate 1 ea
Transcription Factor NF-κB Competitor dsDNA 1 ea
Transcription Factor NF-κB (human p65) Positive Control 1 ea
Transcription Factor NF-κB (p65) Primary Antibody 1 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
Wash Buffer Concentrate (400X) 5 mL
Size Price Quantity Subtotal
96 wells $410.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample NF-κB (p65) Transcription Factor Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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