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Thioredoxin Reductase Assay Kit

Cayman Chemical Item Number 10007892

TrxR Assay Kit

Thioredoxin Reductase Assay Kit

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Description

Cayman’s Thioredoxin Reductase (TrxR) Assay Kit provides a convenient method for quantifying mammalian TrxR activity from colorimetric tissue homogenates and cell lysates in a 96 well plate format. In this assay, TrxR uses NADPH to reduce DTNB to 5-thio-2-nitrobenzoic acid (TNB) which absorbs strongly at 405-414 nm.1,2 The kit includes all reagents needed to assay mammalian TrxR activity. Measurement of TrxR activity in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction.3

1 Smith, A.D., Morris, V.C., and Levander, O.A. Rapid determination of glutathione peroxidase and thioredoxin reductase activities using a 96-well microplate format: Comparison to standard cuvette-based assays. Int J Vitam Nutr Res 71(1) 87-92 (2001).

2 Hill, K.E., McCollum, G.W., and Burk, R.F. Determination of thioredoxin reductase activity in rat liver supernatant. Anal Biochem 253 123-125 (1997).

3 Smith, A.D., Guidry, C.A., Morris, V.C., et al. Aurothioglucose inhibits murine thioredoxin reductase activity in vivo. J Nutr 129 194-198 (1999).

Synonyms
  • TrxR Assay Kit
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Mustacich, D., and Powis, G. Thioredoxin reductase. Biochem J 346 1-8 (2000).

Hill, K.E., McCollum, G.W., and Burk, R.F. Determination of thioredoxin reductase activity in rat liver supernatant. Anal Biochem 253 123-125 (1997).

Gromer, S., Arscott, L.D., Williams, C.H., et al. Human placenta thioredoxin reductase. Isolation of the selenoenzyme, steady kinetics, and inhibition by therapeutic gold compounds. J Biol Chem 273(32) 20096-20101 (1998).

Smith, A.D., Guidry, C.A., Morris, V.C., et al. Aurothioglucose inhibits murine thioredoxin reductase activity in vivo. J Nutr 129 194-198 (1999).

Smith, A.D., Morris, V.C., and Levander, O.A. Rapid determination of glutathione peroxidase and thioredoxin reductase activities using a 96-well microplate format: Comparison to standard cuvette-based assays. Int J Vitam Nutr Res 71(1) 87-92 (2001).

Becker, K., Gromer, S., Schirmer, R.H., et al. Thioredoxin reductase as a pathophysiological factor and drug target. Eur J Biochem 267 6118-6125 (2000).

Turanov, A.A., Su, D., and Gladyshev, V.N. Mouse mitochondrial thioredoxin reductase: Characterization of alternative cytosolic forms and cellular targets. J Biol Chem 281(32) 22953-22963 (2006).

Sun, Q., Wu, Y., Zappacosta, F., et al. Redox regulation of cell signaling by selenocysteine in mammalian thioredoxin reductases. J Biol Chem 274(35) 24522-24530 (1999).

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10007892-96WELL
TrxR Assay Buffer (10X) 1 ea
Thioredoxin Reductase Control 1 ea
TrxR Inhibitor 2 × 1 ea
TrxR NADPH 2 × 1 ea
TrxR DTNB 1 ea
TrxR DMSO 1 ea
96-Well Solid Plate (Colorimetric Assay)
Size Price Quantity Subtotal
96 wells $178.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Thioredoxin Reductase Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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