Cayman Chemical | Thioredoxin Reductase Assay Kit | Description • TrxR Assay Kit

10007892 Thioredoxin Reductase Assay Kit

TrxR Assay Kit

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Cayman’s Thioredoxin Reductase (TrxR) Assay Kit provides a convenient method for quantifying mammalian TrxR activity from colorimetric tissue homogenates and cell lysates in a 96 well plate format. In this assay, TrxR uses NADPH to reduce DTNB to 5-thio-2-nitrobenzoic acid (TNB) which absorbs strongly at 405-414 nm.^1,2 The kit includes all reagents needed to assay mammalian TrxR activity. Measurement of TrxR activity in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction.³

1 Smith, A.D., Morris, V.C., Levander, O.A. Rapid determination of glutathione peroxidase and thioredoxin reductase activities using a 96-well microplate format: Comparison to standard cuvette-based assays. Int J Vitam Nutr Res 71(1) 87-92 (2001).

2 Hill, K.E., McCollum, G.W., Burk, R.F. Determination of thioredoxin reductase activity in rat liver supernatant. Anal Biochem 253 123-125 (1997).

3 Smith, A.D., Guidry, C.A., Morris, V.C., et al. Aurothioglucose inhibits murine thioredoxin reductase activity in vivo. J Nutr 129 194-198 (1999).

	Download Kit Booklet 431 Kb PDF
	Download Material Safety Data Sheet (MSDS) 73 Kb PDF
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	• US Letter paper 8½″ × 11″
	• A4 paper 21cm × 29.7cm

Purchase 10007892 Thioredoxin Reductase Assay Kit

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Warning This product is not for human or veterinary use.

Pricing updated 2010-03-20.
Prices are subject to change without notice.

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