Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday.
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Epoxyeicosatrienoic acid (EpETrE; EET) metabolites of arachidonic acid such as 11(12)-EET and 14(15)-EET have been identified as endothelium derived hyperpolarizing factors with vasodilator activity.1 Soluble epoxide hydrolase (sEH) catalyzes the conversion of EETs to the corresponding dihydroxy eicosatrienoic acids (DiHETrEs; DHETs) thereby diminishing their activity. CUDA is an inhibitor of sEH exhibiting IC50 values of 11.1 nM and 112 nM for the mouse and human enzymes, respectively.2 In COS-7 cells, 10 µM CUDA blocks conversion of 1 µM 14,15-EET to 14,15-DHET by 94%. CUDA activates peroxisome proliferator-activated receptor α (PPARα) 8-fold at a concentration of 10 µM but exhibits no affect on PPARδ or PPARγ3
1
Fleming, I. Cytochrome P450 epoxygenases as EDHF synthase(s). Pharmacol Res49525-533(2004).
2
Morisseau, C., Goodrow, M.H., Newman, J.W., et al. Structural refinement of inhibitors of urea-based soluble epoxide hydrolases. Biochem Pharmacol631599-1608(2002).
3
Fang, X., Hu, S., Watanabe, T., et al. Activation of peroxisome proliferator-activated receptor α by substituted urea-derived soluble epoxide hydrolase inhibitors. J Pharmacol Exp Ther314(1)260-270(2005).