Source: recombinant C-terminal hexahistidine-tagged protein expressed in E. coli · M_r: ~37 kDa · Protein concentration: 0.8 mg/ml · Human prostaglandin F synthase (PGFS) is a ~37 kDa monomeric protein with high sequence similarity to aldo-keto reductase family members.¹ It exhibits two related primary activities, catalyzing the isomerization of PGH₂ to PGF_2α (PGH₂ 9,11-endoperoxide reductase activity), as well as the formation of 11β-PGF_2α from PGD₂ (PGD₂ 11-ketoreductase activity) in the presence of NADPH. The enzyme also exhibits reductase activities toward carbonyl compounds such at nitroacetophenone, nitrobenzaldehyde, and 9,10-phenanthrene quinine (PQ).² Cayman’s human recombinant PGFS has been expressed and purified from an E.coli overexpression system. Enzyme activity was determined by monitoring the change in absorbance at 340 nm using PGD₂ as the substrate and NADPH as the cofactor (PGD₂ 11-ketoreductase activity).

1 Urade, Y., Watanabe, K., and Hayaishi, O. Prostaglandin D, E, and F synthases. J Lipid Mediat Cell Signal 12 257-273 (1995).

2 Suzuki, T., Fuji, Y., Miyano, M., et al. cDNA cloning, expression, and mutagenesis study of liver-type prostaglandin F synthase. J Biol Chem 274 241-248 (1999).

Urade, Y., Watanabe, K., and Hayaishi, O. Prostaglandin D, E, and F synthases. J Lipid Mediat Cell Signal 12 257-273 (1995).

Suzuki, T., Fuji, Y., Miyano, M., et al. cDNA cloning, expression, and mutagenesis study of liver-type prostaglandin F synthase. J Biol Chem 274 241-248 (1999).

Komoto, J., Yamada, T., Watanabe, K., et al. Crystal structure of human prostaglandin F synthase (AKR1C3). Biochemistry 43 2188-2198 (2004).

Lovering, A.L., Ride, J.P., Bunce, C.M., et al. Crystal structures of prostaglandin D₂ 11-ketoreductase (AKR1C3) in complex with the nonsteroidal anti-inflammatory drugs flufenamic acid and indomethacin. Cancer Res 64 1802-1810 (2004).

Watanabe, K., Yoshida, R., Shimizu, T., et al. Enzymatic formation of prostaglandin F_2α from prostaglandin H₂ and D₂. Purification and properties of prostaglandin F synthetase from bovine lung. J Biol Chem 260(11) 7035-7041 (1985).

Komoto, J., Yamada, T., Watanabe, K., et al. Prostaglandin F_2α formation from prostaglandin H₂ by prostaglandin F synthase (PGFS): Crystal structure of PGFS containing bimatoprost. Biochemistry 45(7) 1987-1996 (2006).

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