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15-hydroxy Prostaglandin Dehydrogenase (human recombinant)

Cayman Chemical Item Number 10007950

15-hydroxy PGDH

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Description

Source: active human recombinant N-terminal GST-tagged protein expressed in E. coli · Mr: ~55 kDa · NAD+-dependent 15-hydroxy prostaglandin dehydrogenase (15-PGDH) catalyzes the oxidation of PGs to 15-keto metabolites that have greatly reduced biological activity.1 Human NAD+-dependent 15-PGDH is a 266 amino acid protein with a molecular weight of 29 kDa and is expressed in multiple tissues including lung, placenta, and kidney.2 The enzyme belongs to a large family of short-chain alcohol dehydrogenases, which exhibit amino acid identity ranging from 15-30%.3,4 Recent studies indicate 15-PGDH may play a role as a tumor suppressor. Studies have shown an inverse correlation between 15-PGDH expression and cell proliferation in colon, breast, and thyroid cells.5,6,7 15-PGDH specific activity was determined using PGE2 as the substrate and NAD+ as the cofactor and was calculated after subtracting the mass contribution form the GST tag. The reaction was inhibited using CAY10397.

1 Hansen, H.S. 15-Hydroxyprostaglandin dehydrogenase. A review. Prostaglandins 12 647-679 (1976).

2 Ensor, C.M., Yang, J., Okita, R.T., et al. Cloning and sequence analysis of the cDNA for human placental NAD+-dependent 15-hydroxyprostaglandin dehydrogenase. J Biol Chem 265 14888-14891 (1990).

3 Jörnvall, H., Persson, B., Krook, M., et al. Short-chain dehydrogenases/reductases (SDR). Biochemistry 34 6003-6013 (1995).

4 Krook, M., Marekov, L., and Jörnvall, H. Purification and structural characterization of placental NAD+-linked 15-hydroxyprostaglandin dehydrogenase. The primary structure reveals the enzyme to belong to the short-chain alcohol dehydrogenase family. Biochemistry 29 738-743 (1990).

5 Myung, S., Rerko, R.M., Yan, M., et al. 15-Hydroxyprostaglandin dehydrogenase is an in vivo suppressor of colon tumorigenesis. Proc Natl Acad Sci USA 103 12098-12102 (2006).

6 Quidville, V., Segond, N., Lausson, S., et al. 15-Hydroxyprostaglandin-dehydrogenase is involved in anti-proliferative effect of non-steroidal anti-inflammatory drugs COX-1 inhibitors on a human medullary thyroid carcinoma cell line. Prostaglandins Other Lipid Mediat 81 14-30 (2006).

7 Wolf, I., O'Kelly, J., Rubinek, T., et al. 15-Hydroxyprostaglandin dehydrogenase is a tumor suppressor of human breast cancer. Cancer Res 66 7818-7823 (2006).

Synonyms
  • 15-hydroxy PGDH
Formulation A solution in 50 mM Tris-HCl, pH 7.5, containing 1 mM EDTA, 1 mM DTT, and 30% glycerol
Purity ≥95% by SDS-PAGE
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Hansen, H.S. 15-Hydroxyprostaglandin dehydrogenase. A review. Prostaglandins 12 647-679 (1976).

Jörnvall, H., Persson, B., Krook, M., et al. Short-chain dehydrogenases/reductases (SDR). Biochemistry 34 6003-6013 (1995).

Wolf, I., O'Kelly, J., Rubinek, T., et al. 15-Hydroxyprostaglandin dehydrogenase is a tumor suppressor of human breast cancer. Cancer Res 66 7818-7823 (2006).

Quidville, V., Segond, N., Lausson, S., et al. 15-Hydroxyprostaglandin-dehydrogenase is involved in anti-proliferative effect of non-steroidal anti-inflammatory drugs COX-1 inhibitors on a human medullary thyroid carcinoma cell line. Prostaglandins Other Lipid Mediat 81 14-30 (2006).

Myung, S., Rerko, R.M., Yan, M., et al. 15-Hydroxyprostaglandin dehydrogenase is an in vivo suppressor of colon tumorigenesis. Proc Natl Acad Sci USA 103 12098-12102 (2006).

Krook, M., Marekov, L., and Jörnvall, H. Purification and structural characterization of placental NAD+-linked 15-hydroxyprostaglandin dehydrogenase. The primary structure reveals the enzyme to belong to the short-chain alcohol dehydrogenase family. Biochemistry 29 738-743 (1990).

Ensor, C.M., Yang, J., Okita, R.T., et al. Cloning and sequence analysis of the cDNA for human placental NAD+-dependent 15-hydroxyprostaglandin dehydrogenase. J Biol Chem 265 14888-14891 (1990).

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Size Price Quantity Subtotal
25 µg $105.00 $0.00
50 µg $200.00 $0.00
100 µg $378.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-26. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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