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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

20S Proteasome Assay Kit

Cayman Chemical Item Number 10008041

20S Proteasome Assay Kit

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Description

The proteasome is a multicatalytic proteinase complex that is involved in the selective degradation of intracellular proteins. The 20S proteasome is the proteolytic core particle of a large protein degradation complex, the 26S proteasome. Proteasome inhibitors exhibit anti-inflammatory and antiproliferative effects, evidence that the proteasome may be an important drug target for the treatment for cancer and inflammatory diseases. Cayman’s 20S Proteasome Assay Kit employs a specific 20S substrate, SUC-LLVY-AMC which, upon cleavage by the active enzyme, generates a highly fluorescent product with an emission wavelength at 480 nm. The kit is easy to use and can be easily adapted to high throughput screening for therapeutic compounds regulating activation of the 20S proteasome.
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

McCormack, T.A., Cruikshank, A.A., Grenier, L., et al. Kinetic studies of the branched chain amino acid preferring peptidase activity of the 20S proteasome: Development of a continuous assay and inhibition by tripeptide aldehydes and clasto-lactacystin β-lactone. Biochemistry 37 7792-7800 (1998).

Reidlinger, J., Pike, A.M., Savory, P.J., et al. Catalytic properties of 26 S and 20 S proteasomes and radiolabeling of MB1, LMP7, and C7 subunits associated with trypsin-like and chymotrypsin-like activities. J Biol Chem 272(40) 24899-24905 (1997).

Stein, R.L., Melandri, F., and Dick, L. Kinetic characterization of the chymotryptic activity of the 20S proteasome. Biochemistry 35 3899-3908 (1996).

Rivett, A.J., Savory, P.J., and Djaballah, H. Multicatalytic endopeptidase complex: Proteasome. Methods Enzymol 244 331-350 (1994).

DeMartino, G.N., and Slaughter, C.A. The proteasome, a novel protease regulated by multiple mechanisms. J Biol Chem 274(32) 22123-22126 (1999).

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10008041-1EA
20S Proteasome Positive Control 1 ea
20S Proteasome Substrate (SUC-LLVY-AMC) 1 ea
Cell-Based Assay Epigallocatechin Gallate (EGCG) 1 ea
20S Proteasome Assay Buffer 1 ea
20S Proteasome Lysis Buffer 1 ea
96-Well Solid Plate (black) with lid
Size Price Quantity Subtotal
1 ea $269.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample 20S Proteasome Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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