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10008051 Acid Phosphatase Assay Kit
Let Cayman analyze your samples for you. See EIA Service for details and availability.
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Acid phosphatases (APs) are members of the hydrolase class of enzymes catalyze the hydrolysis of orthophosphate monoesters under acidic conditions.1 The concentration of human APs undergo pronounced changes in particular diseases, resulting in unusually high or low concentrations. Thus, AP levels are often used as clinical markers of disease. The levels of prostate acid phosphatase (PAP) have long been used as an indicator of prostate cancer, while an increased level of tartrate resistant acid phosphatase (TRAP) is often indicative of bone disease.1,2 Cayman’s Acid Phosphatase Assay Kit provides a convenient method for detecting AP activity in plasma, serum, urine, and semen. The assay utilizes para-nitrophenyl phosphate (pNPP) as a chromogenic substrate for the enzyme. In the first step, AP dephosphorylates pNPP. In the second step, the phenolic OH-group is deprotonated under alkaline conditions resulting in p-nitrophenolate that yields an intense yellow color which can be measured at 405-414 nm.3 The kit provides all reagents needed to assay AP activity, including L-tartrate, an inhibitor of non-tartrate resistant APs.
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1
Bull, H., Murray, P.G., Thomas, D., et al. Acid phosphatases. J Clin Pathol: Mol Pathol 55, 65-72 (2002).
2
Yam, L.T. Clinical significance of the human acid phosphatases. The American Journal of Medicine 56 604-616 (1974).
3
Hudson, P.B., Brendler, H., Scott, W.W. A simple method for the determination of serum acid phosphatase. J Urol 58 89-92 (1947).
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Purchase 10008051 Acid Phosphatase Assay Kit
Pricing is for North America only. Other customers should contact a distributor in their region.
This product is also available to buy in bulk quantities.
Please contact our
Sales Department
for a quote or to purchase.
Cayman strives to be a reliable biochemical reagent vendor by providing the best possible products and services.
To ask for assistance with one of our products please contact our Technical Help staff.
Warning This product is not for human or veterinary use.
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Pricing updated 2008-10-11.
Prices are subject to change without notice.
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