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S-NEPC Exclusive

Cayman Chemical Item Number 10008609

S-NEPC

See more	Colorimetric Assay (52) Enzyme Activity (151) Endocrinology (337) Hypertension (97)

Description

Cytochrome P450 metabolites of arachidonic acid, such as 11(12)-EpETrE and 14(15)-EpETrE have been identified as endothelium derived hyperpolarizing factors with vasodilator activity.¹ Soluble epoxide hydrolase (sEH) catalyzes the conversion of EpETrEs to the corresponding DiHETrEs thereby diminishing their activity.² Inhibitors of sEH may therefore have clinical utility for treating hypertension and systemic inflammation.^3,4 S-NEPC is a colorimetric substrate used to measure sEH activity.^5,6 It also is a substrate for Glutathione S-transferase, microsomal epoxide hydrolase and porcine liver carboxylesterase.⁵ Hydrolysis of S-NEPC by sEH yields 4-nitrophenol which can be quantified spectrophotometrically at 405 nm. S-NEPC is adaptable for use in 96-well microwell plate readers.

1 Fleming, I. Cytochrome P450 epoxygenases as EDHF synthase(s). Pharmacol Res 49 525-533 (2004).

2 Morisseau, C., Goodrow, M.H., Newman, J.W., et al. Structural refinement of inhibitors of urea-based soluble epoxide hydrolases. Biochem Pharmacol 63 1599-1608 (2002).

3 Imig, J.D., Zhao, X., Zaharis, C.Z., et al. An orally active epoxide hydrolase inhibitor lowers blood pressure and provides renal protection in salt-sensitive hypertension. Hypertension 46(2) 975-981 (2005).

4 Schmelzer, K.R., Kubala, L., Newman, J.W., et al. Soluble epoxide hydrolase is a therapeutic target for acute inflammation. Proc Natl Acad Sci USA 102(28) 9772-9777 (2005).

5 Dietze, E.C., Kuwano, E., and Hammock, B.D. Spectrophotometric substrates for cytosolic epoxide hydrolase. Anal Biochem 216 176-187 (1994).

6 Jones, P.D., Wolf, N.M., Morisseau, C., et al. Fluorescent substrates for soluble epoxide hydrolase and application to inhibition studies. Anal Biochem 343 66-75 (2005).

Formal Name	4-nitrophenyl-2S,3S-epoxy-3 phenylpropyl carbonate
Molecular Formula	C₁₆H₁₃NO₆
Formula Weight	315.3
Formulation	A crystalline solid
Purity	≥98%
λ_max	213, 266 nm
Stability	2 years
Storage	-20°C
Shipping	Room temperature in continental US; may vary elsewhere
SMILES	Copy SMILES to clipboard O=C(OC1=CC=C([N+]([O-])=O)C=C1)OC[C@H]2[C@@H](O2)C3=CC=CC=C3

Background Reading

Morisseau, C., Goodrow, M.H., Newman, J.W., et al. Structural refinement of inhibitors of urea-based soluble epoxide hydrolases. Biochem Pharmacol 63 1599-1608 (2002).

Schmelzer, K.R., Kubala, L., Newman, J.W., et al. Soluble epoxide hydrolase is a therapeutic target for acute inflammation. Proc Natl Acad Sci USA 102(28) 9772-9777 (2005).

Jones, P.D., Wolf, N.M., Morisseau, C., et al. Fluorescent substrates for soluble epoxide hydrolase and application to inhibition studies. Anal Biochem 343 66-75 (2005).

Dietze, E.C., Kuwano, E., and Hammock, B.D. Spectrophotometric substrates for cytosolic epoxide hydrolase. Anal Biochem 216 176-187 (1994).

Imig, J.D., Zhao, X., Zaharis, C.Z., et al. An orally active epoxide hydrolase inhibitor lowers blood pressure and provides renal protection in salt-sensitive hypertension. Hypertension 46(2) 975-981 (2005).

Fleming, I. Cytochrome P450 epoxygenases as EDHF synthase(s). Pharmacol Res 49 525-533 (2004).

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S-NEPC is available in the following screening library:

Bio-active Lipid I Screening Library (96-Well)

Size	Price	Quantity	Subtotal
1 mg	$15.00		$0.00
5 mg	$68.00		$0.00
10 mg	$120.00		$0.00
25 mg	$263.00		$0.00
Bulk	Contact
		Cart Total	$0.00

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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