|
10008882 LDH Cytotoxicity Assay Kit
Lactate Dehydrogenase Cytotoxicity Assay Kit
LDH Cytotoxicity Assay Kit Workbook Links
Cayman offers a variety of analysis tools to assist you.
Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.
Let Cayman analyze your samples for you. See EIA Service for details and availability.
|
Cell death can occur either by apoptosis or by necrosis. Necrosis is accompanied by mitochondrial swelling and increased plasma membrane permeability, whereas apoptosis involves an articulated breakdown of the cell into membrane-bound apoptotic bodies.1 Lactate dehydrogenase (LDH) is a soluble cytosolic enzyme that is released into the culture medium following loss of membrane integrity resulting from either apoptosis or necrosis. LDH activity, therefore, can be used as an indicator of cell membrane integrity and serves as a general means to assess cytotoxicity resulting from chemical compounds or environmental toxic factors. Cayman’s LDH Cytotoxicity Assay Kit measures LDH activity present in the culture medium using a coupled two-step reaction. In the first step, LDH catalyzes the reduction of NAD+ to NADH and H+ by oxidation of lactate to pyruvate. In the second step of the reaction, diaphorase uses the newly-formed NADH and H+ to catalyze the reduction of a tetrazolium salt (INT) to highly-colored formazan which absorbs strongly at 490-520 nm.
|
|
1
Bonfoco, E., Krainc, D., Ankarcrona, M., et al. Apoptosis and necrosis: Two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures. Proc Natl Acad Sci USA 92 7162-7166 (1995).
|
Purchase 10008882 LDH Cytotoxicity Assay Kit
Pricing is for North America only. Other customers should contact a distributor in their region.
This product is also available to buy in bulk quantities.
Please contact our
Sales Department
for a quote or to purchase.
Cayman strives to be a reliable biochemical reagent vendor by providing the best possible products and services.
To ask for assistance with one of our products please contact our Technical Help staff.
Warning This product is not for human or veterinary use.
|
Pricing updated 2010-03-20.
Prices are subject to change without notice.
|
|
|
|
|
