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TBARS Assay KitCayman Chemical Item Number 10009055 Thiobarbituric Acid Reactive Substances Assay Kit 
TBARS Assay Kit
Description
Oxidative stress in the cellular environment results in the formation of highly reactive and unstable lipid hydroperoxides. Decomposition of the unstable peroxides derived from polyunsaturated fatty acids results in the formation of malondialdehyde (MDA), which can be quantified colorimetrically following its controlled reaction with thiobarbituric acid. The measurement of these 'Thiobarbituric Acid Reactive Substances' (TBARS) is a well-
1 Yagi, K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods Mol Biol 108 101-106 (1998). 2 Armstrong, D., and Browne, R. The analysis of free radicals, lipid peroxides, antioxidant enzymes and compounds to oxidative stress as applied to the clinical chemistry laboratory. Free Radicals in Diagnostic Medicine 366 43-58 (1994).
Background ReadingDawn-Linsley, M., Ekinci, F.J., Ortiz, D., et al. Monitoring thiobarbituric acid- Scoccia, A.E., Molinuevo, M.S., McCarthy, A.D., et al. A simple method to assess the oxidative susceptibility of low density lipoproteins. BMC Clin Pathol 1 (2001). Richard, M., Portal, B., Meo, J., et al. Malondialdehyde kit evaluated for determining plasma and lipoprotein fractions that react with thiobarbituric acid. Clin Chem 38(5) 704-709 (1992). Draper, H.H., Squires, E.J., Mahmoodi, H., et al. A comparative evaluation of thiobarbituric acid methods for the determination of malondialdehyde in biological materials. Free Radic Biol Med 15 353-363 (1993). Ohkawa, H., Ohishi, N., and Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95 351-358 (1979). Yagi, K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods Mol Biol 108 101-106 (1998). Armstrong, D., and Browne, R. The analysis of free radicals, lipid peroxides, antioxidant enzymes and compounds to oxidative stress as applied to the clinical chemistry laboratory. Free Radicals in Diagnostic Medicine 366 43-58 (1994). Wang, L., Tsai, A., and Hsu, P. Substrate binding is the rate- |
This product is also available to buy in bulk quantities. Please contact our Sales Department for a quote or to purchase. Pricing updated 2012-02-10. Prices are subject to change without notice. To ask for assistance with one of our products please contact a Technical Support Scientist. Warning This product is not for human or veterinary use. You may be eligible to receive a free sample TBARS Assay Kit under the Cayman Challenge program. Let Cayman analyze your samples for you. See EIA Service for details and availability. Related ProductsShow all 20 Hide all but first 3Downloads
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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins. |
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