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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Caspase-3 Fluorescence Assay Kit

Cayman Chemical Item Number 10009135

Caspase-3 Fluorescence Assay Kit

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Description

Caspase-3 plays a central role in the execution of apoptosis. Activation of caspase-3 is commonly used as a biomarker for assessment of apoptosis and for understanding mechanisms of apoptosis induction. Cayman’s caspase-3 fluorescence assay kit employs a specific caspase-3 substrate, N-Ac-DEVD-N'-MC-R110, which upon cleavage by active caspase-3, generates a highly fluorescent product that can be measured using excitation and emission wavelengths of 485 and 535 nm, respectively.1 The kit is simple to use and can be easily adapted to high throughput screening for therapeutic compounds that may regulate activation of caspase-3. Active caspase-3 is included in the kit as a positive control or as a quantitative standard. The caspase-3 inhibitor, N-Ac-Asp-Glu-Val-Asp-CHO, is also included in the kit for verifying the specificity of the substrate.

1 Wang, Z., Liao, J., and Diwu, Z. N-DEVD-N'-morpholinecarbonyl-rhodamine 110: Novel caspase-3 fluorogenic substrates for cell-based apoptosis assay. Bioorg Med Chem Lett 15 2335-2338 (2005).

Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Wang, Z., Liao, J., and Diwu, Z. N-DEVD-N'-morpholinecarbonyl-rhodamine 110: Novel caspase-3 fluorogenic substrates for cell-based apoptosis assay. Bioorg Med Chem Lett 15 2335-2338 (2005).

Budihardjo, I., Oliver, H., Lutter, M., et al. Biochemical pathways of caspase activation during apoptosis. Annu Rev Cell Dev Biol 15 269-290 (1999).

Bonfoco, E., Krainc, D., Ankarcrona, M., et al. Apoptosis and necrosis: Two distinct events induced, respectively, by mild and intense insults with N-methyl-D-aspartate or nitric oxide/superoxide in cortical cell cultures. Proc Natl Acad Sci USA 92 7162-7166 (1995).

Green, D.R., and Reed, J.C. Mitochondria and apoptosis. Science 281 1309-1312 (1998).

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10009135-1EA
Active Caspase-3 Substrate N-Ac-DEVD-N'-MC-R110 1 ea
Active Caspase-3 Standard 1 ea
Caspase-3 Inhibitor N-Ac-Asp-Glu-Val-Asp-CHO 1 ea
Caspase-3 Dithiothreitol (1M) 1 ea
Caspase-3 Assay Buffer 1 ea
Cell-Based Assay Lysis Buffer 1 ea
Size Price Quantity Subtotal
96 wells $198.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Caspase-3 Fluorescence Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

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