Antigen: phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser^218,222 of human MEK1/2 · Host: rabbit · Application(s): WB · MAP kinase kinase 1 (MEK1) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation.^1,2 This pathway also plays a key role in synaptic plasticity in the brain.³ Activated MEK1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase.^4,5,6

1 Ahn, N.G. The MAP kinase cascade. Discovery of a new signal transduction pathway.. Mol Cell Biochem 127-128 201-209 (1993).

2 Chong, H., Vikis, H.G., and Guan, K.L. Mechanisms of regulating the Raf kinase family.. Cell Signal 15 463-469 (2003).

3 Adams, J.P., and Sweatt, J.D. Molecular psychology: Roles for the ERK MAP kinase cascade in memory.. Annu Rev Pharmacol Toxicol 42 135-163 (2002).

4 Mansour, S.J., Resing, K.A., Candi, J.M., et al. Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: Determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis. J Biochem 116 304-314 (1994).

5 Kyriakis, J.M., Brautigan, D.L., Ingebritsen, T.S., et al. pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity.. J Biol Chem 266(16) 10043-10046 (1991).

6 Seger, R., Ahn, N.G., Boulton, T.G., et al. Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: Implications for their mechanism of activation.. Proc Natl Acad Sci USA 88 6142-6146 (1991).

Ahn, N.G. The MAP kinase cascade. Discovery of a new signal transduction pathway.. Mol Cell Biochem 127-128 201-209 (1993).

Seger, R., Ahn, N.G., Boulton, T.G., et al. Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophosphorylation on both tyrosine and threonine residues: Implications for their mechanism of activation.. Proc Natl Acad Sci USA 88 6142-6146 (1991).

Kyriakis, J.M., Brautigan, D.L., Ingebritsen, T.S., et al. pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity.. J Biol Chem 266(16) 10043-10046 (1991).

Chong, H., Vikis, H.G., and Guan, K.L. Mechanisms of regulating the Raf kinase family.. Cell Signal 15 463-469 (2003).

Mansour, S.J., Resing, K.A., Candi, J.M., et al. Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: Determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis. J Biochem 116 304-314 (1994).

Adams, J.P., and Sweatt, J.D. Molecular psychology: Roles for the ERK MAP kinase cascade in memory.. Annu Rev Pharmacol Toxicol 42 135-163 (2002).

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