Sphingosine Kinase 2 (human recombinant)

Cayman Chemical Item Number 10009237

SPHK 2; SK2

Description

Source: active human recombinant N-terminal His-tagged protein from Sf9 cells · M_r: 69.5 kDa · Sphingosine kinase (SPHK) is an important enzyme in the sphingolipid metabolic pathway. SPHKs phosphorylate sphingolipid D-erythro-sphingosine to yield sphingosine-1-phosphate (S1P). To date, two isoforms of SPHK, SPHK 1 and SPHK 2, have been identified and characterized from mammalian cells.^1,2 SKs, through the formation of S1P, have been implicated in a diverse range of cellular processes, including cell proliferation, apoptosis, calcium homeostasis, angiogenesis, and vascular maturation.³ Thus SPHKs are potential therapeutic targets for the control of cancer, inflammation, and other diseases.^4,3

1 Liu, H., Sugiura, M., Nava, V.E., et al. Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform. J Biol Chem 275(26) 19513-19520 (2000).

2 Nava, V.E., Lacana, E., Poulton, S., et al. Functional characterization of human sphingosine kinase-1. FEBS Lett 473 81-84 (2000).

3 Leclercq, T.M., and Pitson, S.M. Cellular signalling by sphingosine kinase and sphingosine 1-phosphate. IUBMB Life 58 467-472 (2006).

4 Taha, T.A., Hannun, Y.A., and Obeid, L.M. Sphingosine kinase: Biochemical and cellular regulation and role in disease. J Biochem Mol Biol 39(2) 113-131 (2006).

Synonyms

SPHK 2
SK2

Formulation

20 µg in 25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween 20, 50% glycerol, and 3mM DTT

Purity

>80% by SDS-PAGE

Stability

6 months

Storage

-80°C

Shipping

Dry ice in continental US; may vary elsewhere

Background Reading

Leclercq, T.M., and Pitson, S.M. Cellular signalling by sphingosine kinase and sphingosine 1-phosphate. IUBMB Life 58 467-472 (2006).

Liu, H., Sugiura, M., Nava, V.E., et al. Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform. J Biol Chem 275(26) 19513-19520 (2000).

Nava, V.E., Lacana, E., Poulton, S., et al. Functional characterization of human sphingosine kinase-1. FEBS Lett 473 81-84 (2000).

Taha, T.A., Hannun, Y.A., and Obeid, L.M. Sphingosine kinase: Biochemical and cellular regulation and role in disease. J Biochem Mol Biol 39(2) 113-131 (2006).

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