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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

SREBP-2 Blocking Peptide

Cayman Chemical Item Number 10009266

SREBF2; Sterol Regulatory Element-binding Transcription Factor 2; Sterol Regulatory Element-binding Protein-2

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Description

Peptide sequence: human SREBP-2 protein amino acids 455-469 (SPLLDDAKVKDEPDS) · To be used in conjunction with Cayman’s SREBP-2 polyclonal antibody (Catalog No. 10007663) to block protein-antibody complex formation during immunochemical analysis of SREBP-2 protein. · SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism.1 Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids.1 SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway.2 SREBP-2 is ubiquitously detected in various tissues.3 Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol.4 Cayman’s SREBP-2 polyclonal antibody detects both precursor and active forms of the protein in tissues and cells such as liver, brown fat, testis, Hep-G2 cells, and human fibroblast. The apparent molecular weight on SDS-PAGE may be higher than the calculated molecular weight (about 126 kDa) due to glycosylation of the protein.5

1 Brown, M.S., and Goldstein, J.L. The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89 331-340 (1997).

2 Sakai, J., Nohturfft, A., Goldstein, J.L., et al. Cleavage of sterol regulatory element-binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies. J Biol Chem 273(10) 5785-5793 (1998).

3 Iizuka, K., Bruick, R.K., Liang, G., et al. Deficiency of carbohydrate response element-binding protein (ChREBP) reduces lipogenesis as well as glycolysis. Proc Natl Acad Sci USA 101(19) 7281-7286 (2004).

4 Smith, L.H., Petrie, M.S., Morrow, J.D., et al. 7The sterol response element binding protein regulates cyclooxygenase-2 gene expression in endothelial cells. J Lipid Res 46 862-871 (2005).

5 Hua, X., Sakai, J., Ho, Y.K., et al. Hairpin orientation of sterol regulatory element-binding protein-2 in cell membranes as determined by protease protection. J Biol Chem 270(49) 29422-29427 (1995).

Synonyms
  • SREBF2
  • Sterol Regulatory Element-binding Transcription Factor 2
  • Sterol Regulatory Element-binding Protein-2
Formulation 200 µg of peptide in 200 µl TBS, pH 7.4, containing 0.1% BSA and 0.02% sodium azide
Stability 2 years
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Brown, M.S., and Goldstein, J.L. The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89 331-340 (1997).

Iizuka, K., Bruick, R.K., Liang, G., et al. Deficiency of carbohydrate response element-binding protein (ChREBP) reduces lipogenesis as well as glycolysis. Proc Natl Acad Sci USA 101(19) 7281-7286 (2004).

Sakai, J., Nohturfft, A., Goldstein, J.L., et al. Cleavage of sterol regulatory element-binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies. J Biol Chem 273(10) 5785-5793 (1998).

Smith, L.H., Petrie, M.S., Morrow, J.D., et al. 7The sterol response element binding protein regulates cyclooxygenase-2 gene expression in endothelial cells. J Lipid Res 46 862-871 (2005).

Hua, X., Sakai, J., Ho, Y.K., et al. Hairpin orientation of sterol regulatory element-binding protein-2 in cell membranes as determined by protease protection. J Biol Chem 270(49) 29422-29427 (1995).

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Pricing updated 2012-02-12. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

Related Products

Cholesterol Assay Kit
SREBP-2 Polyclonal Antibody
SREBP-2 Transcription Factor Assay Kit
SREBP-2 Western Ready Control
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