See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Cysteinyl Leukotriene Express EIA Kit

Cayman Chemical Item Number 10009291

CysLT Express ELISA Kit

Cysteinyl Leukotriene Express EIA Kit

See analysis tools…

Cysteinyl Leukotriene Express EIA Kit Analysis Tools

Cayman offers a variety of analysis tools to assist you.

Pre-configured for analysis on MyAssays.com
See more

Description

Limit of detection: 80% B/B0: 20 pg/ml · Sensitivity: 50% B/B0: 80 pg/ml · Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid via the 5-lipoxygenase (5-LO) pathway in leukocytes. LTC4, LTD4, and LTE4 are collectively referred to as cysteinyl leukotrienes (CysLTs). LTC4 and LTD4 are potent mediators of asthma and hypersensitivity. They induce bronchoconstriction, increase microvascular permeability, and are vasoconstrictors of coronary arteries. The biological activity of LTE4 is much lower in most systems studied, but its presence reflects the prior existence of LTC4 and LTD4. Cayman’s CysLT Express EIA Kit is a competitive assay that can be used for measurement of all three primary CysLTs in urine, culture media, and other sample matrices. The EIA typically displays an IC50 (50% B/B0) of approximately 80 pg/ml and a detection limit (80% B/B0) of approximately 20 pg/ml. This sensitive assay takes about three hours to complete.
Synonyms
  • CysLT Express ELISA Kit
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere
Specificity
N-methyl Leukotriene C4 124%
Leukotriene C4 100%
Leukotriene D4 100%
Leukotriene E4 65%
Leukotriene B4 <0.01%
5,6-DiHETE <0.01%
5,15-DiHETE <0.01%
5-HETE <0.01%
12-HETE <0.01%
15-HETE <0.01%
Arachidonic Acid <0.01%
Prostaglandin D2 <0.01%
Prostaglandin E2 <0.01%
Show all 13 Hide all but first 3

Background Reading

Miyata, A., Hara, S., Yokoyama, C., et al. Molecular cloning and expression of human prostacyclin synthase. Biochem Biophys Res Commun 200 1728-1734 (1994).

Matsumoto, S., Hayashi, Y., Kinoshita, I., et al. Immunoaffinity purification of prostaglandin E2 and leukotriene C4 prior to radioimmunoassay: Application to human synovial fluid. Ann Clin Biochem 30(Part I) 60-68 (1993).

Samuelsson, B., Dahlén, S., Lindgren JÅ, et al. Leukotrienes and lipoxins: Structures, biosynthesis, and biological effects. Science 237 1171-1176 (1987).

Campbell, B.J., Baker, S.F., Shukla, S.D., et al. Bioconversion of leukotriene D4 by lung dipeptidase. Biochim Biophys Acta 1042 107-112 (1990).

Dahlén, S., Björk, J., Hedqvist, P., et al. Leukotrienes promote plasma leakage and leukocyte adhesion in postcapillary venules: In vivo effects with relevance to the acute inflammatory response. Proc Natl Acad Sci USA 78 3887-3891 (1981).

Liu, J., García-Cardeña, G., and Sessa, W.C. Palmitoylation of endothelial nitric oxide synthase is necessary for optimal stimulated release of nitric oxide. Implications for caveolae localization. Biochemistry 35 13277-13281 (1996).

Maxey, K.M., Maddipati, K.R., and Birkmeier, J. Interference in enzyme immunoassays. J Clin Immunoassay 15 116-120 (1992).

Piper, P.J. Formation and actions of leukotrienes. Physiol Rev 64 744-761 (1984).

Maclouf, J., Corvazier, E., and Wang, Z. Development of a radioimmunoassay for prostaglandin D2 using an antiserum against 11-methoxime prostaglandin D2. Prostaglandins 31 123-132 (1986).

Hardy, G., Boizel, R., Bessard, J., et al. Urinary leukotriene E4 excretion is increased in type 1 diabetic patients a quantification by liquid chromatography-tandem mass spectrometry. Prostaglandins Other Lipid Mediat 78 291-299 (2005).

Mita, H., Turikisawa, N., Yamada, T., et al. Quantification of leukotriene B4 glucuronide in human urine. Prostaglandins Other Lipid Mediat 83 42-49 (2007).

Cai, C., Yang, J., Hu, S., et al. Relationship between urinary cysteinyl leukotriene E4 levels and clinical response to antileukotriene treatment in patients with asthma. Lung 185 105-112 (2007).

Show all 12 Hide all but first 3
10009291-480well
Cysteinyl Leukotriene Express EIA Monoclonal Antibody 500 dtn
Cysteinyl Leukotriene Express AChE Tracer 500 dtn
Immunoassay Buffer A Concentrate (10X) 4 × 10 mL
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 5 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
Ellman's Reagent 6 × 250 dtn
Wash Buffer Concentrate (400X) 12.5 mL
Cysteinyl Leukotriene EIA Standard 1 ea
10009291-96solid
Cysteinyl Leukotriene Express EIA Monoclonal Antibody 100 dtn
Cysteinyl Leukotriene Express AChE Tracer 100 dtn
Immunoassay Buffer A Concentrate (10X) 2 × 10 mL
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 1 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
Ellman's Reagent 3 × 100 dtn
Wash Buffer Concentrate (400X) 5 mL
Cysteinyl Leukotriene EIA Standard 1 ea
10009291-480solid
Cysteinyl Leukotriene Express EIA Monoclonal Antibody 500 dtn
Cysteinyl Leukotriene Express AChE Tracer 500 dtn
Immunoassay Buffer A Concentrate (10X) 4 × 10 mL
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Solid Plate 5 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
Ellman's Reagent 6 × 250 dtn
Wash Buffer Concentrate (400X) 12.5 mL
Cysteinyl Leukotriene EIA Standard 1 ea
10009291-96well
Cysteinyl Leukotriene Express EIA Monoclonal Antibody 100 dtn
Cysteinyl Leukotriene Express AChE Tracer 100 dtn
Immunoassay Buffer A Concentrate (10X) 2 × 10 mL
Precoated (Goat Anti-Mouse IgG) EIA 96-Well Strip Plate 1 ea
96-Well Cover Sheet
Polysorbate 20 1 ea
Ellman's Reagent 3 × 100 dtn
Wash Buffer Concentrate (400X) 5 mL
Cysteinyl Leukotriene EIA Standard 1 ea
Size Price Quantity Subtotal
96 solid wells $335.00 $0.00
96 strip wells $335.00 $0.00
480 solid wells $1,256.00 $0.00
480 strip wells $1,256.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

  • All 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution.
  • Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Cysteinyl Leukotriene Express EIA Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

Related Products

Cysteinyl Leukotriene EIA Kit
HAMI3379 Exclusive
Leukotriene C4 EIA Kit
Leukotriene E4 EIA Kit
SPE Cartridges (C-18)
UltraPure Water
Show all 6 Hide all but first 3

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?