Malachite Green Phosphate Assay Kit
Cayman Chemical Item Number 10009325
Malachite Green Phosphate Assay Kit
Description
Cayman’s Malachite Green Phosphate Assay Kit provides a fast, reproducible, colorimetric method for measuring inorganic free phosphate in aqueous solutions. The assay method is based on the formation of a complex between malachite green molybdate and free orthophosphate that absorbs at 620-640 nm.1,2 Applications for this assay include quantification of phosphorylation and phosphate release from protein phosphatase substrates.3 This assay is a reliable and suitable means of detecting and quantifying minimal amounts of inorganic free phosphate and is amenable to high-throughput screening applications.4 The assay is formatted to a 96-well format, but could easily be modified for use in 384-well or cuvette-based assays.
1
D'Angelo, E., Crutchfield, J., and Vandiviere, M. Rapid, sensitive, microscale determination of phosphate in water and soil. J Environ Qual 30 2206-2209 (2001).
2
O'Toole, M., Lau, K.T., Shepherd, R., et al. Determination of phosphate using a highly sensitive paired emitter-detector diode photometric flow detector. Anal Chim Acta 597 290-294 (2007).
3
Maehama, T., Taylor, G.S., Slama, J.T., et al. A sensitive assay for phosphoinositide phosphatases. Anal Biochem 279 248-250 (2000).
4
Attin, T., Becker, K., Hannig, C., et al. Suitability of a malachite green procedure to detect minimal amounts of phosphate dissolved in acidic solutions. Clin Oral Investig 9(3) 203-207 (2005).
| Stability |
6 months |
| Storage |
4°C |
| Shipping |
Wet ice
in continental US; may vary elsewhere
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Background Reading
D'Angelo, E., Crutchfield, J., and Vandiviere, M. Rapid, sensitive, microscale determination of phosphate in water and soil. J Environ Qual 30 2206-2209 (2001).
O'Toole, M., Lau, K.T., Shepherd, R., et al. Determination of phosphate using a highly sensitive paired emitter-detector diode photometric flow detector. Anal Chim Acta 597 290-294 (2007).
Attin, T., Becker, K., Hannig, C., et al. Suitability of a malachite green procedure to detect minimal amounts of phosphate dissolved in acidic solutions. Clin Oral Investig 9(3) 203-207 (2005).
Maehama, T., Taylor, G.S., Slama, J.T., et al. A sensitive assay for phosphoinositide phosphatases. Anal Biochem 279 248-250 (2000).
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Pricing updated 2012-02-12.
Prices are subject to change without notice.
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Warning This product is not for human or veterinary use.
You may be eligible to receive a free sample Malachite Green Phosphate Assay Kit under the Cayman Challenge program.
Let Cayman analyze your samples for you. See EIA Service for details and availability.
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