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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

Aldehyde Reactive Probe (trifluoroacetate salt)

Cayman Chemical Item Number 10009350

O-(Biotinylcarbazoylmethyl) Hydroxylamine; ARP (CAS 627090-10-2)

Aldehyde Reactive Probe (trifluoroacetate salt) (CAS 627090-10-2)

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Description

DNA is continually damaged by endogenous and environmental agents leading to the formation of abasic (apurinic/apyrimidinic, AP) sites that are disruptive to DNA synthesis. Aldehyde Reactive Probe (ARP) is a biotinylated reagent for the detection and quantification of AP sites in damaged DNA. ARP reacts with aldehyde groups formed when reactive oxygen species depurinate DNA, resulting in covalent linkage of biotin to these AP sites.1 The biotin-tagged DNA can then be detected using common avidin-conjugated reporters such as avidin-HRP. The ARP method is highly sensitive, enabling detection of 2.4 AP sites per 1x107 nucleotides of DNA.2

1 Kurisu, S., Miya, T., Terato, H., et al. Quantitation of DNA damage by an aldehyde reactive probe (ARP). Nucleic Acids Res Suppl 1 45-46 (2001).

2 Nakamura, J., Walker, V.E., Upton, P.B., et al. Highly sensitive apurinic/apyrimidinic site assay can detect spontaneous and chemically induced depurination under physiological conditions. Cancer Res 58 222-225 (1998).

Synonyms
  • O-(Biotinylcarbazoylmethyl) Hydroxylamine
  • ARP
Formal Name (6aR)-​hexahydro-​2-​oxo-​2-​[(aminooxy)acetyl]hydrazide,​1H-​thieno[3aS,​4S-​d]imidazole-​4-​pentanoic acid,​ trifluoroacetate salt
CAS Number 627090-10-2
Molecular Formula C12H21N5O4S • C2HF3O2
Formula Weight 445.4
Formulation A crystalline solid
Purity ≥98%
Stability 2 years
Storage -20°C
Shipping Room temperature in continental US; may vary elsewhere
SMILES
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O=C1N([H]​)​[C@@]​2([H]​)​[C@@]​(CS[C@@H]​2CCCCC(N([H]​)​N([H]​)​C(CON)​=O)​=O)​([H]​)​N1[H]​.FC(F)​(C(O)​=O)​F

Background Reading

Nakamura, J., Walker, V.E., Upton, P.B., et al. Highly sensitive apurinic/apyrimidinic site assay can detect spontaneous and chemically induced depurination under physiological conditions. Cancer Res 58 222-225 (1998).

Kurisu, S., Miya, T., Terato, H., et al. Quantitation of DNA damage by an aldehyde reactive probe (ARP). Nucleic Acids Res Suppl 1 45-46 (2001).
Size Price Quantity Subtotal
5 mg $47.00 $0.00
10 mg $89.00 $0.00
25 mg $212.00 $0.00
50 mg $376.00 $0.00
Bulk Contact
Cart Total $0.00

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Pricing updated 2012-02-12. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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