Antigen: peptide from N-terminal region of the α1-subunit of the rat glycine receptor · Host: rabbit · Application(s): WB and IHC (frozen sections) · Glycine is an important inhibitory transmitter in the brain stem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission.¹ The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin.² The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins.^3,4 These receptors have also been extremely useful in studies of synaptic clustering of receptors.⁵ The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor.⁶

1 Schofield, C.M., Jenkins, A., and Harrison, N.L. A highly conserved aspartic acid residue in the signature disulfide loop of the .alpa.1-subunit is a determinant of gating in the glycine receptor. J Biol Chem 278 34079-34083 (2003).

2 Fischer, F., Kneussel, M., Tintrup, H., et al. Reduced synaptic clustering of GABA and glycine receptors in the retina of the gephyrin null mutant mouse. J Comp Neurol 427 634-648 (2000).

3 Beckstead, M.J., Phelan, R., and Mihic, S.J. Antagonism of inhalant and volatile anesthetic enhancement of glycine receptor function. J Biol Chem 276 24959-24964 (2001).

4 Mihic, S.J., Ye, Q., Wick, M.J., et al. Sites of alcohol and volatile anaesthetic action of GABAA and glycine receptors. Nature 389 385-389 (1997).

5 Craig, A.M., and Lichtman, J.W. Getting a bead on receptor movements. Nat Neurosci 4 219-220 (2001).

6 Chatterton, J.E., Awobuluyi, M., Premkumar, L.S., et al. Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits. Nature 415 793-798 (2002).

Schofield, C.M., Jenkins, A., and Harrison, N.L. A highly conserved aspartic acid residue in the signature disulfide loop of the .alpa.1-subunit is a determinant of gating in the glycine receptor. J Biol Chem 278 34079-34083 (2003).

Fischer, F., Kneussel, M., Tintrup, H., et al. Reduced synaptic clustering of GABA and glycine receptors in the retina of the gephyrin null mutant mouse. J Comp Neurol 427 634-648 (2000).

Mihic, S.J., Ye, Q., Wick, M.J., et al. Sites of alcohol and volatile anaesthetic action of GABAA and glycine receptors. Nature 389 385-389 (1997).

Chatterton, J.E., Awobuluyi, M., Premkumar, L.S., et al. Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits. Nature 415 793-798 (2002).

Craig, A.M., and Lichtman, J.W. Getting a bead on receptor movements. Nat Neurosci 4 219-220 (2001).

Beckstead, M.J., Phelan, R., and Mihic, S.J. Antagonism of inhalant and volatile anesthetic enhancement of glycine receptor function. J Biol Chem 276 24959-24964 (2001).

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