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Tyrosine Hydroxylase (Phospho-Ser40) Polyclonal Antibody

Cayman Chemical Item Number 10009414

TH

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Description

Antigen: phosphopeptide corresponding to amino acid residues surrounding phospho-Ser40 of rat TH · Host: rabbit · Application(s): WB, IF, and IHC · Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease, and drug abuse.1,2,3 TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling.4,5,6 The activity of TH is also regulated by phosphorylation.7,8,9 Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

1 Kish, S.J., Kalasinsky, K.S., Derkach, P., et al. Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24 561-567 (2001).

2 Zhu, M.Y., Klimek, V., Haycock, J.W., et al. Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Methods 99 37-44 (2000).

3 Zhu, M.Y., Klimek, V., Diley, G.E., et al. Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46 1275-1286 (1999).

4 Witkovsky, P., Gabriel, R., Haycock, J.W., et al. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19 105-116 (2000).

5 Salvatore, M.F., Waymire, J.C., and Haycock, J.W. Depolarization-stimulated catecholamine biosynthesis: Involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79 349-350 (2001).

6 Dunkley, P.R., Bobrovskaya, L., Graham, M.E., et al. Tyrosine hydroxylase phosphorylation: Regulation and consequences. J Neurochem 91 1025-1043 (2004).

7 Haycock, J.W., Ahn, N.G., Cobb, M.H., et al. ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ. Proc Natl Acad Sci USA 89 2365-2369 (1992).

8 Haycock, J.W., Bennett, W.F., George, R.J., et al. Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine. J Biol Chem 257 13699-13703 (1982).

9 Jedynak, J.P., Ali, S.F., Haycock, J.W., et al. Acute administration of cocaine regulates the phosphorylation of serine-19,-31 and -40 in tyrosine hydroxylase. J Neurochem 82 382-388 (2002).

Synonyms
  • TH
Formulation Affinity-purified antibody
Stability 1 year
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere
Specificity
mammalian TH +
non-mammalian TH +

Background Reading

Salvatore, M.F., Waymire, J.C., and Haycock, J.W. Depolarization-stimulated catecholamine biosynthesis: Involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ. J Neurochem 79 349-350 (2001).

Jedynak, J.P., Ali, S.F., Haycock, J.W., et al. Acute administration of cocaine regulates the phosphorylation of serine-19,-31 and -40 in tyrosine hydroxylase. J Neurochem 82 382-388 (2002).

Haycock, J.W., Ahn, N.G., Cobb, M.H., et al. ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ. Proc Natl Acad Sci USA 89 2365-2369 (1992).

Dunkley, P.R., Bobrovskaya, L., Graham, M.E., et al. Tyrosine hydroxylase phosphorylation: Regulation and consequences. J Neurochem 91 1025-1043 (2004).

Kish, S.J., Kalasinsky, K.S., Derkach, P., et al. Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24 561-567 (2001).

Zhu, M.Y., Klimek, V., Haycock, J.W., et al. Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Methods 99 37-44 (2000).

Haycock, J.W., Bennett, W.F., George, R.J., et al. Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine. J Biol Chem 257 13699-13703 (1982).

Witkovsky, P., Gabriel, R., Haycock, J.W., et al. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J Chem Neuroanat 19 105-116 (2000).

Zhu, M.Y., Klimek, V., Diley, G.E., et al. Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46 1275-1286 (1999).

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Pricing updated 2012-05-25. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

Related Products

Tyrosine Hydroxylase (Phospho-Ser19) Polyclonal Antibody
Tyrosine Hydroxylase (Phospho-Ser31) Polyclonal Antibody

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