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ChREBP DBD (human recombinant)

Cayman Chemical Item Number 10009524

GenBank Accession No. BC012925; Carbohydrate Response Element-binding Protein DNA Binding Domain; WS-bHLH; Williams-Beuren Syndrome Chromosome Region 14

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Description

Source: human recombinant N-terminal GST-tagged protein expressed in E. coli · Mr: ~38.3 kDa · Carbohydrate response element-binding protein (ChREBP) is a member of the basic helix-loop-helix leucine zipper transcription factor family. Under conditions of low glucose, ChREBP is phosphorylated which sequesters the transcription factor in the cytoplasm. Under conditions of elevated glucose, one of its metabolites, xylulose 5-phosphate, activates Protein Phosphatase 2A (PP2A) which dephosphorylates ChREBP at Ser196. Dephosphorylated (Ser196) ChREBP translocates into the nucleus where it is further dephosphorylated at Thr666 by PP2A. The second dephosphorylation allows ChREBP to bind to the carbohydrate response element (ChRE) within the promoter of the L-type pyruvate kinase (L-PK) gene, as well as a subset of lipogenic enzymes.1,2,3 Activation of ChREBP by high glucose in the liver occurs independent of insulin. The inactivation of ChREBP has been proposed as a potential method for reducing obesity and also improving glucose tolerance.1,4 Cayman · s ChREBP DBD contains amino acids 648-741 of the full length protein fused to GST at the N-terminus.

1 Iizuka, K., Miller, B., and Uyeda, K. Deficiency of a carbohydrate-activated transcription factor, ChREBP, prevents obesity and improves plasma glucose control in leptin deficient (ob/ob) mice. Am J Physiol Endocrinol Metab 291 E358-E364 (2006).

2 Kawaguchi, T., Takenoshita, M., Kabashima, T., et al. Glucose and cAMP regulate the L-type pyruvate kinase gene by phosphorylation/dephosphorylation of the carbohydrate response element binding protein. Proc Natl Acad Sci USA 98(24) 13710-13715 (2001).

3 Ishii, S., Iizuka, K., Miller, B.C., et al. Carbohydrate response element binding protein directly promotes lipogenic enzyme gene transcription. Proc Natl Acad Sci USA 101(44) 15597-15602 (2004).

4 Yamashita, H., Takenoshita, M., Sakurai, M., et al. A glucose-responsive transcription factor that regulates carbohydrate metabolism in the liver. Proc Natl Acad Sci USA 98(16) 9116-9121 (2001).

Synonyms
  • GenBank Accession No. BC012925
  • Carbohydrate Response Element-binding Protein DNA Binding Domain
  • WS-bHLH
  • Williams-Beuren Syndrome Chromosome Region 14
Formulation 50 mM sodium phosphate pH 7.2, 100 mM sodium chloride, 1 mM DTT, and 40% glycerol
Purity >85% by SDS-PAGE
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Iizuka, K., Miller, B., and Uyeda, K. Deficiency of a carbohydrate-activated transcription factor, ChREBP, prevents obesity and improves plasma glucose control in leptin deficient (ob/ob) mice. Am J Physiol Endocrinol Metab 291 E358-E364 (2006).

Yamashita, H., Takenoshita, M., Sakurai, M., et al. A glucose-responsive transcription factor that regulates carbohydrate metabolism in the liver. Proc Natl Acad Sci USA 98(16) 9116-9121 (2001).

Kawaguchi, T., Takenoshita, M., Kabashima, T., et al. Glucose and cAMP regulate the L-type pyruvate kinase gene by phosphorylation/dephosphorylation of the carbohydrate response element binding protein. Proc Natl Acad Sci USA 98(24) 13710-13715 (2001).

Ishii, S., Iizuka, K., Miller, B.C., et al. Carbohydrate response element binding protein directly promotes lipogenic enzyme gene transcription. Proc Natl Acad Sci USA 101(44) 15597-15602 (2004).

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Size Price Quantity Subtotal
5 µg $72.00 $0.00
10 µg $137.00 $0.00
25 µg $324.00 $0.00
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Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

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Pricing updated 2012-05-25. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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