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sPLA2 (human recombinant Type V)

Cayman Chemical Item Number 10009563

Secretory Phospholipase A2 (Group V); gVPLA2

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Description

Source: active human recombinant protein expressed in E. coli · Mr: 14 kDa · Phospholipase A2 (PLA2) catalyzes the hydrolysis of fatty acids at the sn-2 position of glycerophospholipids. PLA2 (Type V) is a secretory PLA2 (sPLA2) of approximately 14 kDa and is one of the isoforms in the growing list of the PLA2 enzyme family.1 This enzyme, rather than the sPLA2 (Type II), is responsible for arachidonic acid mobilization leading to prostaglandin production in macrophages and mast cells.2,3,4 Consistent with this role, sPLA2 (Type V) is associated with the golgi apparatus, nuclear envelope, and plasma membrane in murine bone marrow-derived mast cells.5 sPLA2 (Type V) has been cloned from a variety of species including human, mouse, and rat.6,7,8 The enzyme is expressed in heart, lung, placenta, and spleen, as well as P388D1 macrophages and mast cells.2,3,8 The specific activity of Cayman’s sPLA2 (human recombinant Type V) was established using 1,2-bis(heptanoylthio) Glycerophosphocholine (Cayman item no. 62235) as the substrate. The reaction was inhibited by thioetheramide-PC (Cayman item no. 62750).

1 Balsinde, J., Winstead, M.V., and Dennis, E. Phospholipase A2 regulation of arachidonic acid mobilization. FEBS Lett 531 2-6 (2002).

2 Balboa, M.A., Balsinde, J., Winstead, M.V., et al. Novel group V phospholipase A2 involved in arachidonic acid mobilization in murine P388D1 macrophages. J Biol Chem 271 32381-32384 (1996).

3 Reddy, S.T., Winstead, M.V., Tischfield, J.A., et al. Analysis of the secretory phospholipase A2 that mediates prostaglandin production in mast cells. J Biol Chem 272 13591-13596 (1997).

4 Bingham, C.O., Murakami, M., Fujishima, H., et al. A heparin-sensitive phospholipase A2 and prostaglandin endoperoxide synthase-2 are functionally linked in the delayed phase of prostaglandin D2 generation in mouse bone marrow-derived mast cells. J Biol Chem 271 25936-25944 (1996).

5 Bingham, C.O., Fijneman, R.J.A., Friend, D.S., et al. Low molecular weight group IIA and group V phospholipase A2 enzymes have different intracellular locations in mouse bone marrow-derived mast cells. J Biol Chem 274 31476-31484 (1999).

6 Chen, J., Engle, S.J., Seilhamer, J.J., et al. Cloning and recombinant expression of a novel human low molecular weight Ca2+-dependent phospholipase A2. J Biol Chem 269 2365-2368 (1994).

7 Tischfield, J.A., Xia, Y., Shih, D.M., et al. Low-molecular-weight, calcium-dependent phospholipase A2 genes are linked and map to homologous chromosome regions in mouse and human. Genomics 32 328-333 (1996).

8 Chen, J., Engle, S.J., Seilhamer, J.J., et al. Cloning, expression and partial characterization of a novel rat phospholipase A2. Biochim Biophys Acta 1215 115-120 (1994).

Synonyms
  • Secretory Phospholipase A2 (Group V)
  • gVPLA2
Formulation A solution in 50 mM Tris-HCl, pH 8.0, containing 100 mM sodium chloride, 50 mM calcium chloride, and 20% glycerol
Purity ≥95%
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Balsinde, J., Winstead, M.V., and Dennis, E. Phospholipase A2 regulation of arachidonic acid mobilization. FEBS Lett 531 2-6 (2002).

Balboa, M.A., Balsinde, J., Winstead, M.V., et al. Novel group V phospholipase A2 involved in arachidonic acid mobilization in murine P388D1 macrophages. J Biol Chem 271 32381-32384 (1996).

Reddy, S.T., Winstead, M.V., Tischfield, J.A., et al. Analysis of the secretory phospholipase A2 that mediates prostaglandin production in mast cells. J Biol Chem 272 13591-13596 (1997).

Tischfield, J.A., Xia, Y., Shih, D.M., et al. Low-molecular-weight, calcium-dependent phospholipase A2 genes are linked and map to homologous chromosome regions in mouse and human. Genomics 32 328-333 (1996).

Chen, J., Engle, S.J., Seilhamer, J.J., et al. Cloning, expression and partial characterization of a novel rat phospholipase A2. Biochim Biophys Acta 1215 115-120 (1994).

Chen, J., Engle, S.J., Seilhamer, J.J., et al. Cloning and recombinant expression of a novel human low molecular weight Ca2+-dependent phospholipase A2. J Biol Chem 269 2365-2368 (1994).

Bingham, C.O., Fijneman, R.J.A., Friend, D.S., et al. Low molecular weight group IIA and group V phospholipase A2 enzymes have different intracellular locations in mouse bone marrow-derived mast cells. J Biol Chem 274 31476-31484 (1999).

Bingham, C.O., Murakami, M., Fujishima, H., et al. A heparin-sensitive phospholipase A2 and prostaglandin endoperoxide synthase-2 are functionally linked in the delayed phase of prostaglandin D2 generation in mouse bone marrow-derived mast cells. J Biol Chem 271 25936-25944 (1996).

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Size Price Quantity Subtotal
10 µg $134.00 $0.00
25 µg $318.00 $0.00
50 µg $603.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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sPLA2 (Type V) Inhibitor Screening Assay Kit
Thioetheramide-PC
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