Application(s): positive control for WB · Source: human recombinant C-terminal His-tagged protein expressed in E. coli · M_r: 36 kDa · Monoacylglycerol lipase (MGL) hydrolyzes 2-AG to terminate its biological actions and works consecutively with hormone-sensitive lipase (HSL) to mobilize fatty acids from the triglyceride stores of adipocytes.^1,2 MGL has a molecular weight of ~33 kDa and exhibits a high degree of homology at the amino acid level among human, mouse, and rat.^3,4,2,1 MGL is expressed in a variety of tissues such as kidney, spleen, heart, liver, testis, stomach, brain, lung, and adrenal gland, with most abundant expression in skeletal muscle and adipose tissue.

1 Karlsson, M., Reue, K., Xia, Y., et al. Exon-intron organization and chromosomal localization of the mouse monoglyceride lipase gene. Gene 272 11-18 (2001).

2 Dinh, T.P., Carpenter, D., Leslie, F.M., et al. Brain monoglyceride lipase participating in endocannabinoid inactivation. Proc Natl Acad Sci USA 99(16) 10819-10824 (2002).

3 Karlsson, M., Contreras, J.A., Hellman, U., et al. cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases. J Biol Chem 272 27218-27223 (1997).

4 Dinh, T.P., Freund, T.F., and Piomelli, D. A role for monoglyceride lipase in 2-arachidonoylglycerol inactivation. Chem Phys Lipids 121 149-158 (2002).

Karlsson, M., Reue, K., Xia, Y., et al. Exon-intron organization and chromosomal localization of the mouse monoglyceride lipase gene. Gene 272 11-18 (2001).

Paolini, J.F., Mitchel, Y.B., Reyes, R., et al. Effects of Laropiprant on nicotinic acid-induced flushing in patients with dyslipidemia. Am J Cardiol 101 625-630 (2008).

Karlsson, M., Contreras, J.A., Hellman, U., et al. cDNA cloning, tissue distribution, and identification of the catalytic triad of monoglyceride lipase. Evolutionary relationship to esterases, lysophospholipases, and haloperoxidases. J Biol Chem 272 27218-27223 (1997).

Dinh, T.P., Carpenter, D., Leslie, F.M., et al. Brain monoglyceride lipase participating in endocannabinoid inactivation. Proc Natl Acad Sci USA 99(16) 10819-10824 (2002).

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