Glucose Assay Kit

Cayman Chemical Item Number 10009582

Glucose Assay Kit

Description

Glucose, a monosaccharide (or simple sugar), is the most important carbohydrate in biology. Transported via the blood stream, it is the primary source of energy for the body’s cells. Glucose level is tightly regulated in the human body. Failure to maintain blood glucose in the normal range leads to conditions of persistently high (hyperglycemia) or low (hypoglycemia) blood sugar. Diabetes mellitus, characterized by persistent hyperglycemia, is the most prominent disease related to failure of blood sugar regulation. Cayman’s Glucose Assay Kit provides a simple, reproducible, and sensitive tool for assaying glucose in plasma, serum, and urine. The glucose assay uses the glucose oxidase-peroxide reaction for the determination of glucose concentrations. In this assay, glucose is oxidized to δ-gluconolactone with concomitant reduction of the flavin adenine dinucleotide (FAD)-dependent enzyme glucose oxidase. The reduced form of glucose oxidase is regenerated to its oxidized form by molecular oxygen to produce hydrogen peroxide. Finally, with horseradish peroxidase as a catalyst, hydrogen peroxide reacts with 3,5-dichloro-2-hydroxybenzenesulfonic acid and 4-aminoantipyrine to generate a pink dye with an optimal absorption at 514 nm.

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Bernfeld, P. In amylase, α and β. 1 149-158 (1955).

Sugiura, M., and Hirano, K. A new colorimetric method for determination of serum glucose. Clin Chim Acta 75 387-391 (1977).

Slein, M.W. D-glucose, determination with hexokinase and glucose-6-phosphate dehydrrogenase. Methods of Enzymatic Analysis 1 117-123 (1963).

Frost, L.D. Glucose assays revisited: Experimental determination of the glucose concentration in honey. Chem Educ 9(4) 239-241 (2004).

Carroll, J., Smith, N., and Babson, A.L. A colorimetric serum glucose determination using hexokinase and glucose-6-phosphate dehydrogenase. Biochem Med 4 171-180 (1970).

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10009582-192WELL

Glucose Assay Standard

1 ea

Glucose Assay Buffer

1 ea

Glucose Colorimetric Enzyme Mixture

4 × 1 ea

96-Well Solid Plate (Colorimetric Assay)