Cayman Chemical | nNOS Western Ready Control | Description • NOS I; ncNOS

10009632 nNOS Western Ready Control

NOS I; ncNOS

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Application(s): positive control for WB · Source: human recombinant C-terminal His-tagged protein expressed in Sf21 cells · M_r: 153 kDA (His-tagged), 150 kDa (native) · Nitric oxide Synthase (NOS) catalyzes the oxidation of arginine to nitric oxide (NO) and citrulline. Three distinct isoforms of NOS have been described having nomenclature based on the tissue source from which they were originally cloned. These three isoforms are neuronal/brain NOS (nNOS/bNOS/NOS-I), inducible NOS (iNOS/NOS-II), and endothelial NOS (eNOS/NOS-III).^1,2 nNOS is a soluble enzyme found in brain, the peripheral nervous system, and skeletal muscle. ^3,4 An alternately spliced form of nNOS (nNOS · ) containing a 34 amino acid insert has been identified in skeletal muscle.⁵ In neurons, protein-protein interactions with PSD-95 and PSD-93 via the PZD domain at the N-terminus of nNOS localizes the enzyme with NMDA receptors ^6,7 Although nNOS was originally thought to be constitutively expressed, abundant. evidence suggests expression is regulated by a variety of conditions.⁸

1 Michel, T., Xie, Q., Nathan, C. Molecular biological analysis of nitric oxide synthases. (1996).

2 Michel, T., Feron, O. Nitric oxide synthases: Which, where, how, and why?. J Clin Invest 100 2146-2152 (1997).

3 Bredt, D.S., Hwang, P.M., Glatt, C.E., et al. Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase. Nature 351 714-718 (1991).

4 Nakane, M., Schmidt, H.H.H.W., Pollock, J.S., et al. Cloned human brain nitric oxide synthase is highly expressed in skeletal muscle. FEBS Lett 316 175-180 (1993).

5 Silvagno, F., Xia, H., Bredt, D.S. Neuronal nitric-oxide synthase-μ, an alternatively spliced isoform expressed in differentiated skeletal muscle. J Biol Chem 271 11204-11208 (1996).

6 Brenman, J.E., Christopherson, K.S., Craven, S.E., et al. Cloning and characterization of postsynaptic density 93, a nitric oxide synthase interacting protein. J Neurosci 16 7407-7415 (1996).

7 Christopherson, K.S., Bredt, D.S. Nitric oxide in excitable tissues: Physiological roles and disease. J Clin Invest 100 2424-2429 (1997).

8 Wang, Y., Newton, D.C., Marsden, P.A. Neuronal NOS: Gene structure, mRNA diversity, and functional relevance. Critical Reviews in Neurobiology 13 21-43 (1999).

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Pricing updated 2010-03-19.
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