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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

5-Lipoxygenase (human recombinant) Western Ready Control

Cayman Chemical Item Number 10009638

5-LOX; 5-LO

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Description

Application(s): positive control for WB · 5-LO catalyzes the formation of 5(S)-HpETE from arachidonic acid as well as its subsequent conversion to leukotriene A4. 1 5-LO is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages.2 Upon stimulation of the cells, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and cPLA2.3,4 5-LO has a molecular mass of 78 kDa. The enzyme is predominately expressed in peripheral blood leukocytes and also in eosinophils, mast cells, epithelial cells, and alveolar macrophages isolated from the lung.5

1 Shimizu, T., Rådmark, O., and Samuelsson, B. Enzyme with dual lipoxygenase activities catalyzes leukotriene A4 synthesis from arachidonic acid. Proc Natl Acad Sci USA 81 689-693 (1984).

2 Peters-Golden, M. Cell biology of the 5-lipoxygenase pathway. Am J Respir Crit Care Med 157 5227-5232 (1998).

3 Pouliot, M., McDonald, P.P., Krump, E., et al. Colocalization of cytosolic phospholipase A2, 5-lipoxygenase, and 5-lipoxygenase-activating protein at the nuclear membrane of A23187-stimulated human neutrophils. Eur J Biochem 238 250-258 (1996).

4 Brock, T.G., McNish, R.W., Bailie, M.B., et al. Rapid import of cytosolic 5-lipoxygenase into the nucleus of neutrophils after in vivo recruitment and in vitro adherence. J Biol Chem 272 8276-8280 (1997).

5 Komatsu, N., Natsui, K., Ueda, N., et al. Immunohistochemical study on arachidonate 5-lipoxygenase in porcine leukocytes and other tissues. J Histochem Cytochem 39 655-662 (1991).

Synonyms
  • 5-LOX
  • 5-LO
Purity 78 kDa (His-tagged), 74 kDa (native)
Stability 2 years
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Shimizu, T., Rådmark, O., and Samuelsson, B. Enzyme with dual lipoxygenase activities catalyzes leukotriene A4 synthesis from arachidonic acid. Proc Natl Acad Sci USA 81 689-693 (1984).

Peters-Golden, M. Cell biology of the 5-lipoxygenase pathway. Am J Respir Crit Care Med 157 5227-5232 (1998).

Brock, T.G., McNish, R.W., Bailie, M.B., et al. Rapid import of cytosolic 5-lipoxygenase into the nucleus of neutrophils after in vivo recruitment and in vitro adherence. J Biol Chem 272 8276-8280 (1997).

Komatsu, N., Natsui, K., Ueda, N., et al. Immunohistochemical study on arachidonate 5-lipoxygenase in porcine leukocytes and other tissues. J Histochem Cytochem 39 655-662 (1991).

Pouliot, M., McDonald, P.P., Krump, E., et al. Colocalization of cytosolic phospholipase A2, 5-lipoxygenase, and 5-lipoxygenase-activating protein at the nuclear membrane of A23187-stimulated human neutrophils. Eur J Biochem 238 250-258 (1996).

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This product is available in custom sizes and/or larger quantities.

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Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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