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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

15-hydroxy Prostaglandin Dehydrogenase Western Ready Control

Cayman Chemical Item Number 10009742

15-hydroxy PGDH

15-hydroxy Prostaglandin Dehydrogenase Western Ready Control

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Description

Application(s): positive control for WB · Source: human recombinant N-Terminal GST-Tagged protein expressed in E.coli · Mr: 55 kDa (29 kDa 15-hydroxy PGDH; 26 kDa GST tag) · NAD+-dependent 15-hydroxy prostaglandin dehydrogenase (15-hydroxy PGDH) catalyzes the oxidation of prostaglandins to 15-keto metabolites, which have greatly reduced biological activity.1 Human NAD+-dependent 15-hydroxy PGDH is a 266 amino acid protein with a molecular weight of 29 kDa and is expressed in multiple tissues including lung, placenta, and kidney.2 The enzyme belongs to a large family of short-chain alcohol dehydrogenases, which exhibit amino acid identity ranging from 15-30%.3,4 Recent studies indicate 15-hydroxy PGDH may play a role as a tumor suppressor. Three different studies have shown inverse correlation between 15-hydroxy PGDH expression and cell proliferation in colon, breast, and thyroid cells.5,6,7

1 Hansen, H.S. 15-Hydroxyprostaglandin dehydrogenase. A review. Prostaglandins 12 647-679 (1976).

2 Ensor, C.M., Yang, J., Okita, R.T., et al. Cloning and sequence analysis of the cDNA for human placental NAD+-dependent 15-hydroxyprostaglandin dehydrogenase. J Biol Chem 265 14888-14891 (1990).

3 Jörnvall, H., Persson, B., Krook, M., et al. Short-chain dehydrogenases/reductases (SDR). Biochemistry 34 6003-6013 (1995).

4 Krook, M., Marekov, L., and Jörnvall, H. Purification and structural characterization of placental NAD+-linked 15-hydroxyprostaglandin dehydrogenase. The primary structure reveals the enzyme to belong to the short-chain alcohol dehydrogenase family. Biochemistry 29 738-743 (1990).

5 Myung, S., Rerko, R.M., Yan, M., et al. 15-Hydroxyprostaglandin dehydrogenase is an in vivo suppressor of colon tumorigenesis. Proc Natl Acad Sci USA 103 12098-12102 (2006).

6 Quidville, V., Segond, N., Lausson, S., et al. 15-Hydroxyprostaglandin-dehydrogenase is involved in anti-proliferative effect of non-steroidal anti-inflammatory drugs COX-1 inhibitors on a human medullary thyroid carcinoma cell line. Prostaglandins Other Lipid Mediat 81 14-30 (2006).

7 Wolf, I., O'Kelly, J., Rubinek, T., et al. 15-Hydroxyprostaglandin dehydrogenase is a tumor suppressor of human breast cancer. Cancer Res 66 7818-7823 (2006).

Synonyms
  • 15-hydroxy PGDH
Purity ≥90% by SDS-PAGE
Stability 2 years
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Hansen, H.S. 15-Hydroxyprostaglandin dehydrogenase. A review. Prostaglandins 12 647-679 (1976).

Myung, S., Rerko, R.M., Yan, M., et al. 15-Hydroxyprostaglandin dehydrogenase is an in vivo suppressor of colon tumorigenesis. Proc Natl Acad Sci USA 103 12098-12102 (2006).

Wolf, I., O'Kelly, J., Rubinek, T., et al. 15-Hydroxyprostaglandin dehydrogenase is a tumor suppressor of human breast cancer. Cancer Res 66 7818-7823 (2006).

Quidville, V., Segond, N., Lausson, S., et al. 15-Hydroxyprostaglandin-dehydrogenase is involved in anti-proliferative effect of non-steroidal anti-inflammatory drugs COX-1 inhibitors on a human medullary thyroid carcinoma cell line. Prostaglandins Other Lipid Mediat 81 14-30 (2006).

Krook, M., Marekov, L., and Jörnvall, H. Purification and structural characterization of placental NAD+-linked 15-hydroxyprostaglandin dehydrogenase. The primary structure reveals the enzyme to belong to the short-chain alcohol dehydrogenase family. Biochemistry 29 738-743 (1990).

Ensor, C.M., Yang, J., Okita, R.T., et al. Cloning and sequence analysis of the cDNA for human placental NAD+-dependent 15-hydroxyprostaglandin dehydrogenase. J Biol Chem 265 14888-14891 (1990).

Jörnvall, H., Persson, B., Krook, M., et al. Short-chain dehydrogenases/reductases (SDR). Biochemistry 34 6003-6013 (1995).

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Pricing updated 2012-02-12. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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