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SREBP-2 Western Ready Control

Cayman Chemical Item Number 10009749

Sterol Regulatory Element-binding Transcription Factor 2; Sterol Regulatory Element-binding Protein 2; SREBPF2

See more	Western Blot (557) Proteins (180) Recombinant Proteins (171) Transcription Factors (23) Western Ready Control (27) Atherosclerosis (323) Diabetes (134) Obesity (162)

Description

Application(s): positive control for WB · SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism.¹ Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids.¹ SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway.² SREBP-2 is ubiquitously detected in various tissues.³ Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol.⁴

1 Brown, M.S., and Goldstein, J.L. The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89 331-340 (1997).

2 Sakai, J., Nohturfft, A., Goldstein, J.L., et al. Cleavage of sterol regulatory element-binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies. J Biol Chem 273(10) 5785-5793 (1998).

3 Iizuka, K., Bruick, R.K., Liang, G., et al. Deficiency of carbohydrate response element-binding protein (ChREBP) reduces lipogenesis as well as glycolysis. Proc Natl Acad Sci USA 101(19) 7281-7286 (2004).

4 Smith, L.H., Petrie, M.S., Morrow, J.D., et al. 7The sterol response element binding protein regulates cyclooxygenase-2 gene expression in endothelial cells. J Lipid Res 46 862-871 (2005).

Synonyms	Sterol Regulatory Element-binding Transcription Factor 2 Sterol Regulatory Element-binding Protein 2 SREBPF2
Purity	94 kDa (GST-tagged); 68 kDa (native)
Stability	2 years
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere

Background Reading

Brown, M.S., and Goldstein, J.L. The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell 89 331-340 (1997).

Sakai, J., Nohturfft, A., Goldstein, J.L., et al. Cleavage of sterol regulatory element-binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies. J Biol Chem 273(10) 5785-5793 (1998).

Iizuka, K., Bruick, R.K., Liang, G., et al. Deficiency of carbohydrate response element-binding protein (ChREBP) reduces lipogenesis as well as glycolysis. Proc Natl Acad Sci USA 101(19) 7281-7286 (2004).

Smith, L.H., Petrie, M.S., Morrow, J.D., et al. 7The sterol response element binding protein regulates cyclooxygenase-2 gene expression in endothelial cells. J Lipid Res 46 862-871 (2005).

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Pricing updated 2012-02-12. Prices are subject to change without notice.

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