See personalized New Product recommendations! Get personalized New Product recommendations! Register or Login for personalized New Product recommendations!

Holiday Notification: Cayman Chemical will be closed Monday, May 28, 2012, in observance of the Memorial Day holiday. More…
Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

ChREBP DBD Western Ready Control

Cayman Chemical Item Number 10009753

Carbohydrate Response Element-binding Protein DNA Binding Domain; WS-bHLH; Williams-Beuren Syndrome Chromosome Region 14

See more

Description

Application(s): positive control for WB · Source: human recombinant GST-tagged ChREBP amino acids 648-741 expressed in E. coli · Mr: 36 kDa (GST-tagged), 12.3 kDa (native) · The Carbohydrate Response Element-binding Protein (ChREBP) is a member of the basic helix-loop-helix leucine zipper transcription factor family. Under conditions of low glucose, ChREBP is phosphorylated which sequesters the transcription factor in the cytoplasm. Under conditions of elevated glucose, one of its metabolites, xylulose 5-phosphate activates Protein Phosphatase 2A (PP2A) which dephosphorylates ChREBP at Ser196. Dephosphorylated (Ser196) ChREBP translocates into the nucleus where it is further dephosphorylated at Thr666 by PP2A. The second dephosphorylation allows ChREBP to bind to the carbohydrate response element (CRE) within the promoter of the L-type pyruvate kinase (L-PK) gene, as well as a subset of lipogenic enzymes.1,2,3 Activation of ChREBP by high glucose in the liver occurs independently of insulin concentrations. The inactivation of ChREBP has been proposed as a potential method for reducing obesity and also improving glucose tolerance.1,4

1 Iizuka, K., Miller, B., and Uyeda, K. Deficiency of a carbohydrate-activated transcription factor, ChREBP, prevents obesity and improves plasma glucose control in leptin deficient (ob/ob) mice. Am J Physiol Endocrinol Metab 291 E358-E364 (2006).

2 Kawaguchi, T., Takenoshita, M., Kabashima, T., et al. Glucose and cAMP regulate the L-type pyruvate kinase gene by phosphorylation/dephosphorylation of the carbohydrate response element binding protein. Proc Natl Acad Sci USA 98(24) 13710-13715 (2001).

3 Ishii, S., Iizuka, K., Miller, B.C., et al. Carbohydrate response element binding protein directly promotes lipogenic enzyme gene transcription. Proc Natl Acad Sci USA 101(44) 15597-15602 (2004).

4 Yamashita, H., Takenoshita, M., Sakurai, M., et al. A glucose-responsive transcription factor that regulates carbohydrate metabolism in the liver. Proc Natl Acad Sci USA 98(16) 9116-9121 (2001).

Synonyms
  • Carbohydrate Response Element-binding Protein DNA Binding Domain
  • WS-bHLH
  • Williams-Beuren Syndrome Chromosome Region 14
Stability 2 years
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Iizuka, K., Miller, B., and Uyeda, K. Deficiency of a carbohydrate-activated transcription factor, ChREBP, prevents obesity and improves plasma glucose control in leptin deficient (ob/ob) mice. Am J Physiol Endocrinol Metab 291 E358-E364 (2006).

Yamashita, H., Takenoshita, M., Sakurai, M., et al. A glucose-responsive transcription factor that regulates carbohydrate metabolism in the liver. Proc Natl Acad Sci USA 98(16) 9116-9121 (2001).

Ishii, S., Iizuka, K., Miller, B.C., et al. Carbohydrate response element binding protein directly promotes lipogenic enzyme gene transcription. Proc Natl Acad Sci USA 101(44) 15597-15602 (2004).

Kawaguchi, T., Takenoshita, M., Kabashima, T., et al. Glucose and cAMP regulate the L-type pyruvate kinase gene by phosphorylation/dephosphorylation of the carbohydrate response element binding protein. Proc Natl Acad Sci USA 98(24) 13710-13715 (2001).

Show all 4 Hide all but first 3
Size Price Quantity Subtotal
1 ea $51.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

Related Products

ChREBP Blocking Peptide
ChREBP DBD (human recombinant)
ChREBP Transcription Factor Assay Kit

Downloads

Batch-specific Information

Login to access batch-specific information

Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

Other Resources
Management Team Company Profile Company History ChemAssistant Tools FAQs Cayman Europe Cayman Pharma CaBRI Cayman Gear Press Releases Illustrations and Charts Key Research Area Posters Article Library Analysis Tools Conference Schedule Order Terms Browser Recommendation Privacy Statement Site Map

Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

Toll Free: (800) 364-9897 (USA and Canada Only) · Fax: (734) 971-3640

Copyright 2012 Cayman Chemical Company

Lost password?