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Serum Retinol Binding Protein Western Ready Control Exclusive

Cayman Chemical Item Number 10009754

sRBP4; RBP4

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Description

Application(s): positive control for WB · Source: human recombinant C-terminal His-tagged protein expressed in E. coli · Mr: 26 kDa · Human serum retinol binding protein 4 (sRBP4) binds to one equivalent of vitamin A and is one of the major retinol carriers found in the blood of mammals.1,2 Humans RBP4 is a monomeric 21 kDa β-sheet-rich protein that contains three disulfide bonds and belongs to the lipocalin protein family.3 sRBP4 is synthesized and sequestered in hepatocytes until retinol binding triggers its secretion .3 In plasma, sRBP4 typically forms a 1:1 complex with the 55 kDa tetrameric protein transthyretin (TTR) which prevents RBP from being removed from the plasma by glomerular filtration.4 Studies have shown that sRBP4 is an adipocyte-derived ‘signal’ that may contribute to the pathogenesis of type 2 diabetes. 5,6 Elevation of sRBP4 causes systemic insulin resistance whereas reduction of serum concentrations improves insulin action.5,7,8

1 Noy, N. Retinoid-binding proteins: Mediators of retinoid action. Biochem J 348 481-495 (2000).

2 Xie, Y., Lashuel, H.A., Miroy, G.J., et al. Recombinant human retinol-binding protein refolding, native disulfide formation, and characterization. Protein Expr Purif 14 31-37 (1998).

3 Cowan, S.W., Newcomer, M.E., and Jones, T.A. Crystallographic refinement of human serum retinol binding protein at 2Å resolution. Proteins 8 44-61 (1990).

4 Sivaprasadarao, A., and Findlay, J.B.C. Expression of functional human retinol-binding protein in Escherichia coli using a secretion vector. Biochem J 296 209-215 (1993).

5 Yang, Q., Graham, T.E., Mody, N., et al. Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature 436 356-362 (2005).

6 Muoio, D.M., and Newgard, C.B. A is for adipokine. Nature 436 337-338 (2005).

7 Graham, T.E., Yang, Q., Blüher, M., et al. Retinol-binding protein 4 and insulin resistance in lean, obese, and diabetic subjects. N Engl J Med 354(24) 2552-2563 (2006).

8 Polonsky, K.S. Retinol-binding protein 4, insulin resistance, and type 2 diabetes. N Engl J Med 354(24) 2596-2598 (2006).

Synonyms
  • sRBP4
  • RBP4
Stability 2 years
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Noy, N. Retinoid-binding proteins: Mediators of retinoid action. Biochem J 348 481-495 (2000).

Polonsky, K.S. Retinol-binding protein 4, insulin resistance, and type 2 diabetes. N Engl J Med 354(24) 2596-2598 (2006).

Graham, T.E., Yang, Q., Blüher, M., et al. Retinol-binding protein 4 and insulin resistance in lean, obese, and diabetic subjects. N Engl J Med 354(24) 2552-2563 (2006).

Muoio, D.M., and Newgard, C.B. A is for adipokine. Nature 436 337-338 (2005).

Yang, Q., Graham, T.E., Mody, N., et al. Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature 436 356-362 (2005).

Sivaprasadarao, A., and Findlay, J.B.C. Expression of functional human retinol-binding protein in Escherichia coli using a secretion vector. Biochem J 296 209-215 (1993).

Cowan, S.W., Newcomer, M.E., and Jones, T.A. Crystallographic refinement of human serum retinol binding protein at 2Å resolution. Proteins 8 44-61 (1990).

Xie, Y., Lashuel, H.A., Miroy, G.J., et al. Recombinant human retinol-binding protein refolding, native disulfide formation, and characterization. Protein Expr Purif 14 31-37 (1998).

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Pricing updated 2012-05-25. Prices are subject to change without notice.

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Warning This product is not for human or veterinary use.

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