Antigen: phosphopeptide corresponding to amino acid residues surrounding the Phospho-Tyr¹⁴⁷² of NMDA Receptor NR2B Subunit · Host: rabbit · Application(s): WB · The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s disease, epilepsy and ischemic neuronal death. ^1,2,3 The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS. ^4,5,6 Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. ⁷ Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. ^8,9 Recent work suggests that phosphorylation of Tyr¹⁴⁷² on NR2B may regulate the functional expression of the receptor in LTP and other forms of plasticity. ^10,11

1 Grosshans, D.R. LTP leads to rapid surface expression of NMDA but not AMA receptors in adult rat CA1.. Nat Neurosci 5 27-33 (2002).

2 Wenthold, R.J., Prybylowski, K., Standley, S., et al. Trafficking of NMDA receptors.. Annu Rev Pharmacol Toxicol 43 335-358 (2003).

3 Carroll, R.C., and Zukin, R.S. NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity.. Trends Neurosci 25 571-577 (2002).

4 Lovinger, D.M., White, G., and Weight, F.F. Ethanol inhibits NMDA-activated ion current in hippocampal neurons.. Science 243 1721-1724 (1989).

5 Alvestad, R.M., Grosshans, D.R., Coultrap, S.J., et al. Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278(13) 11020-11025 (2003).

6 Snell, L.D., Nunley, K.R., Lickteig, R.L., et al. Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion.. Mol Brain Res 40 71-78 (1996).

7 Ishii, T., Moriyoshi, K., Sugihara, H., et al. Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits. J Biol Chem 268(4) 2836-2843 (1993).

8 Clayton, D.A., Grosshans, D.R., and Browning, M.D. Aging and surface expression of hippocampal NMDA receptors. J Biol Chem 277(17) 14367-14369 (2002).

9 Clayton, D.A., Mesches, M.H., Alvarez, E., et al. A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat.. J Neurosci 22 3628-3637 (2002).

10 Nakazawa, T., Komai, S., Tezuka, T., et al. Characterization of Fyn-mediated tyrosine phosphorylation sites on GluR.epislon.2 (NR2B) subunit of the N-methyl-D-aspartate receptor. J Biol Chem 276(1) 693-699 (2001).

11 Roche, K.W., Standley, S., McCallum, J., et al. Molecular determinants of NMDA receptor internalization.. Nat Neurosci 4 794-802 (2001).

Snell, L.D., Nunley, K.R., Lickteig, R.L., et al. Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion.. Mol Brain Res 40 71-78 (1996).

Lovinger, D.M., White, G., and Weight, F.F. Ethanol inhibits NMDA-activated ion current in hippocampal neurons.. Science 243 1721-1724 (1989).

Clayton, D.A., Grosshans, D.R., and Browning, M.D. Aging and surface expression of hippocampal NMDA receptors. J Biol Chem 277(17) 14367-14369 (2002).

Grosshans, D.R. LTP leads to rapid surface expression of NMDA but not AMA receptors in adult rat CA1.. Nat Neurosci 5 27-33 (2002).

Wenthold, R.J., Prybylowski, K., Standley, S., et al. Trafficking of NMDA receptors.. Annu Rev Pharmacol Toxicol 43 335-358 (2003).

Carroll, R.C., and Zukin, R.S. NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity.. Trends Neurosci 25 571-577 (2002).

Ishii, T., Moriyoshi, K., Sugihara, H., et al. Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits. J Biol Chem 268(4) 2836-2843 (1993).

Nakazawa, T., Komai, S., Tezuka, T., et al. Characterization of Fyn-mediated tyrosine phosphorylation sites on GluR.epislon.2 (NR2B) subunit of the N-methyl-D-aspartate receptor. J Biol Chem 276(1) 693-699 (2001).

Alvestad, R.M., Grosshans, D.R., Coultrap, S.J., et al. Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278(13) 11020-11025 (2003).

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