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Please feel free to continue placing orders via our website or via fax at 734-971-3640. You may send an email to customer service at custserv@caymanchem.com , or to technical support at techserv@caymanchem.com which we will respond to the next business day. Cayman will resume regular business hours and shipping schedules on Tuesday, May 29, 2012. Thank you for your patience and understanding.

Cholesterol Cell-Based Detection Assay Kit

Cayman Chemical Item Number 10009779

Cholesterol Cell-Based Detection Assay Kit

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Description

The mechanism for the movement of cholesterol from intracellular sites to their ultimate cellular destination is an unresolved question of fundamental importance to cell biology and medicine. Thus, defining mechanisms of intracellular cholesterol transport and identifying the cellular factors involved are therefore of great interest. Cayman’s Cholesterol Cell-Based Detection Assay Kit includes Filipin III, Fixative, and Wash Buffer in a ready to use format. It provides a simple fluorometric method to study mechanisms and biological factors that regulate cholesterol metabolism or movement within cells. A cholesterol trafficking inhibitor, U-18666A, is included as a positive control.
Stability 6 months
Storage -20°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Mukherjee, S., Zha, X., Tabas, I., et al. Cholesterol distribution in living cells: Fluorescence imaging using dehydrogosterol as a fluorescent cholesterol analog. Biophys J 75 1915-1925 (1998).

Soccio, R.E., and Breslow, J.L. Intracellular cholesterol transport. Arterioscler Thromb Vasc Biol 24 1150-1160 (2004).

Drabikowski, W., Lagwinska, E., and Sarzala, M.G. Filipin as a fluorescent probe for the location of cholesterol in the membranes of fragmented sarcoplasmic reticulum. Biochim Biophys Acta 291 61-70 (1973).

Castanho, M.A.R.B., Coutinho, A., and Prieto, M.J.E. Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol. J Biol Chem 267 204-209 (1992).

Miller, R.G. Mini Review. The use and abuse of filipin to localize cholesterol in membranes. Cell Biol Int Rep 8(7) 519-535 (1984).

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10009779-192WELL
Cholesterol Detection Wash Buffer 1 ea
Cholesterol Detection Filipin III 1 ea
Cholesterol Detection Assay Buffer 1 ea
Cell-Based Assay U-18666A 1 ea
Cell-Based Assay Fixative 2 × 1 ea
Size Price Quantity Subtotal
192 wells $153.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Cholesterol Cell-Based Detection Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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