Cholesterol Cell-Based Detection Assay Kit

Cayman Chemical Item Number 10009779

Cholesterol Cell-Based Detection Assay Kit

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Stability	6 months
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere

Description

The mechanism for the movement of cholesterol from intracellular sites to their ultimate cellular destination is an unresolved question of fundamental importance to cell biology and medicine. Thus, defining mechanisms of intracellular cholesterol transport and identifying the cellular factors involved are therefore of great interest. Cayman’s Cholesterol Cell-based Detection Assay Kit includes Filipin III, Fixative, and Wash Buffer in a ready to use format. It provides a simple fluorometric method to study mechanisms and biological factors that regulate cholesterol metabolism or movement within cells. A cholesterol trafficking inhibitor, U18666A, is included as a positive control.

Mukherjee, S., Zha, X., Tabas, I., et al. Cholesterol distribution in living cells: Fluorescence imaging using dehydrogosterol as a fluorescent cholesterol analog. Biophys J 75 1915-1925 (1998).

Soccio, R.E., Breslow, J.L. Intracellular cholesterol transport. Arterioscler Thromb Vasc Biol 24 1150-1160 (2004).

Drabikowski, W., Lagwinska, E., Sarzala, M.G. Filipin as a fluorescent probe for the location of cholesterol in the membranes of fragmented sarcoplasmic reticulum. Biochim Biophys Acta 291 61-70 (1973).

Castanho, M.A.R.B., Coutinho, A., Prieto, M.J.E. Absorption and fluorescence spectra of polyene antibiotics in the presence of cholesterol. J Biol Chem 267 204-209 (1992).

Miller, R.G. Mini Review. The use and abuse of filipin to localize cholesterol in membranes. Cell Biol Int Rep 8(7) 519-535 (1984).

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10009779-192WELL
Cholesterol Detection Wash Buffer	1 ea
Cholesterol Detection Filipin III	1 ea
Cholesterol Detection Assay Buffer	1 ea
Cell-Based Assay U18666A	1 ea
Cell-Based Assay Fixative	2 × 1 ea

Pricing updated 2010-09-02. Prices are subject to change without notice.

Pricing is for North America only. Other customers should contact a distributor in their region.

Cayman strives to be a reliable biochemical reagent vendor by providing the best possible products and services. To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample Cholesterol Cell-Based Detection Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Downloads

	Download Kit Booklet 562 Kb PDF
	Download Material Safety Data Sheet (MSDS) 70 Kb PDF
	Chemical structure definitions are available for many Cayman products. See Chemical Structure Database for details.

Cholesterol Cell-Based Detection Assay Kit