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PtdIns-(4,5)-P2 Binding Protein

Cayman Chemical Item Number 10009815

PIP2; PI(4,5)-P2; Phosphatidylinositol-(4,5)-diphosphate; PLC-δ1-PH Domain

PtdIns-(4,5)-P2 Binding Protein

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Description

Source: recombinant GST-tagged protein purified from E. coli · Mr: ~45.2 kDa · Pleckstrin homology (PH) domains all contain a seven-standard b-sandwich that allows binding to various species of phosphatidylinositol (PtdIns) phosphates.1 Most PH domains bind to PtdIns phosphates with weak affinity and low specificity. However, a small subclass binds specifically and with high-affinity for certain PtdIns phosphates. PtdIns-(4,5)-P2 Binding Protein contains a highly specific PH domain that recognizes and binds PtdIns-(4,5)-P2 (see Figure 1).2 PtdIns phosphates represent a small percentage of total membrane phospholipids. However, they play a critical role in the generation and transmission of cellular signals.3,4 PtdIns-(4,5)-P2 can be phosphorylated by phosphoinositide (PI)-3-kinase to make PtdIns-(3,4,5)-P3 which initiates an intricate signalling cascade that has been implicated in cancer5 PtdIns-(4,5)-P2 binding protein can be used in in vitro assays for the detection of PtdIns-(4,5)-P2 in PI3-kinase and PTEN phosphatase assays.

1 Lemmon, M.A., and Ferguson, K.M. Molecular determinants in pleckstrin homology domains that allow specific recognition of phosphoinositides. Biochem Soc Trans 29(4) 377-384 (2001).

2 Lemmon, M.A. Phosphoinositide recognition domains. Traffic 4 201-213 (2003).

3 Exton, J.H. Regulation of phosphoinositide phospholipases by hormones, neurotransmitters, and other agonists linked to G proteins. Annu Rev Pharmacol Toxicol 36 481-509 (1996).

4 missing reference text

5 Vivanco, I., and Sawyers, C.L. The phosphatidylinositol 3-kinase-AKT pathway in human cancer. Nat Rev Cancer 2 489-501 (2002).

Synonyms
  • PIP2
  • PI(4,5)-P2
  • Phosphatidylinositol-(4,5)-diphosphate
  • PLC-δ1-PH Domain
Formulation A solution in PBS, pH 7.4, containing 20% glycerol
Purity ≥95%
Stability 1 year
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Lemmon, M.A., and Ferguson, K.M. Molecular determinants in pleckstrin homology domains that allow specific recognition of phosphoinositides. Biochem Soc Trans 29(4) 377-384 (2001).

Vivanco, I., and Sawyers, C.L. The phosphatidylinositol 3-kinase-AKT pathway in human cancer. Nat Rev Cancer 2 489-501 (2002).

Majerus, P.W. Inositol phosphate biochemistry. Annu Rev Biochem 61 225-250 (1992).

Lemmon, M.A. Phosphoinositide recognition domains. Traffic 4 201-213 (2003).

Exton, J.H. Regulation of phosphoinositide phospholipases by hormones, neurotransmitters, and other agonists linked to G proteins. Annu Rev Pharmacol Toxicol 36 481-509 (1996).

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Size Price Quantity Subtotal
25 µg $92.00 $0.00
50 µg $175.00 $0.00
100 µg $331.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

Related Products

PLC thio-PIP2 (sodium salt)
PtdIns-(3,4,5)-P3 Binding Protein
PtdIns-(4,5)-P2-biotin (sodium salt)
PtdIns-(4)-P1 Binding Protein
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