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NF-κB (p50) (human recombinant)

Cayman Chemical Item Number 10009818

NF-κB1; Nuclear Factor-κB (p50)

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Description

Application(s): positive control for WB · Source: recombinant enzyme expressed in E. coli and purified out of inclusion bodies · Mr: 74.5 kDa · Specific activity: 40 units/mg · Protein concentration: 0.5 mg/ml · The NF-κB/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-κB 1. Members of this family are responsible for regulating over 150 target genes, including the expression of inflammatory cytokines, chemokines, immunoreceptors, and cell adhesion molecules. Because of this, NF-κB has often been called a 'central mediator of the human immune response.2 Acting as dimers, these transcription factors bind to 10 base pair DNA sequences, collectively called κB sites thereby regulating expression of target genes. The most common Rel/NF-κB dimer in mammals contains (p50)-RelA (p50/p65) heterodimers and is called NF-κB. The importance of Rel/NF-κB transcription factors in human inflammation and certain diseases makes them attractive targets for potential therapeutics.3,4,5

1 Gilmore, T.D. The Rel/NF-κB signal transduction pathway: Introduction. Oncogene 18 6842-6844 (1999).

2 Pahl, H.L. Activators and target genes of Rel/NF-κB transcription factors. Oncogene 18 6853-6866 (1999).

3 Gilroy, D.W., Lawrence, T., Perretti, M., et al. Inflammatory resolution: New opportunities for drug discovery. Nat Rev Drug Discov 3 401-416 (2004).

4 Maeda, S., Hsu, L., Liu, H., et al. Nod2 mutation in Crohn’s disease potentiates NF-κB activity and IL-1β processing. Science 307 734-738 (2005).

5 Clark, A.G., and Debnam, P. Inhibition of glutathione S-transferases from rat liver by S-nitroso-L-glutathione. Biochem Pharmacol 37 3199-3201 (1988).

Synonyms
  • NF-κB1
  • Nuclear Factor-κB (p50)
Formulation A solution in PBS, pH 7.4, containing 5 mM DTT and 20% glycerol
Purity >75%
Stability 6 months
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere

Background Reading

Gilmore, T.D. The Rel/NF-κB signal transduction pathway: Introduction. Oncogene 18 6842-6844 (1999).

Pahl, H.L. Activators and target genes of Rel/NF-κB transcription factors. Oncogene 18 6853-6866 (1999).

Maeda, S., Hsu, L., Liu, H., et al. Nod2 mutation in Crohn’s disease potentiates NF-κB activity and IL-1β processing. Science 307 734-738 (2005).

Arkan, M.C., Hevener, A.L., Greten, F.R., et al. IKK-β links inflammation to obesity-induced insulin resistance. Nat Med 11(2) 191-198 (2005).

Gilroy, D.W., Lawrence, T., Perretti, M., et al. Inflammatory resolution: New opportunities for drug discovery. Nat Rev Drug Discov 3 401-416 (2004).

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Size Price Quantity Subtotal
5 µg $72.00 $0.00
10 µg $137.00 $0.00
25 µg $324.00 $0.00
Bulk Contact
Cart Total $0.00

This product is available in custom sizes and/or larger quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-05-25. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

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