CREB (Phospho-Ser133) Transcription Factor Assay Kit

CREB (Phospho-Ser¹³³) Transcription Factor Assay Kit

Cayman Chemical Item Number 10009846

cAMP-response-element-binding protein (Phospho-Ser¹³³) Transcription Factor Assay Kit

Description

CREB (cAMP-response-element-binding protein) is a transcription factor that binds to cAMP-responsive element (CRE) promoter sites to regulate the transcription of numerous genes involved in metabolic regulation, depression, long term memory, and other physiological processes. Phosphorylation on serine 133 (Ser¹³³) activates CREB to induce transcription of target genes. Diverse stimuli such as growth factors, neurotransmitters, hypoxia, growth factors, UV light, survival signals, and stress signals are some of the known activators of CREB. The signaling systems and mechanisms involved in CREB activation continue to be of interest in academic research and drug discovery programs. Cayman’s CREB (Phospho-Ser¹³³) Transcription Factor Assay is a non-radioactive, sensitive method for detecting CREB DNA binding activity. CREB contained in a nuclear extract or whole cell lysate binds specifically to the DNA cAMP response element immobilized to the wells a 96-well plate. The activated CREB transcription factor complex is detected by addition of a specific primary antibody directed against Phospho-Ser¹³³ on CREB. A secondary antibody conjugated to HRP is used to provide a sensitive colorimetric readout at 450 nm.

Synonyms

cAMP-response-element-binding protein (Phospho-Ser133) Transcription Factor Assay Kit

Stability

6 months

Storage

-80°C

Shipping

Dry ice in continental US; may vary elsewhere

Background Reading

Alberta, J.A., Augers, K.R., Batt, D., et al. Platelet-derived growth factor stimulation of monocyte chemoattractant protein-1 gene expression is mediated by transient activation of the phosphoinositide 3-kinase signal transduction pathway. J Biol Chem 274(43) 31062-31067 (1999).

Johannessen, M., Delghandi, M.P., and Moens, U. What turns CREB on? Cell Signal 16 1211-1227 (2004).

Shaywitz, A.J., and Greenberg, M.E. CREB: A stimulus-induced transcription factor activated by a diverse array of extracellular signals.. Annu Rev Biochem 68 821-861 (1999).

Pennisi, E. Opening the way to gene activity.. Science 275 155-157 (1997).

Silva, A.J., Kogan, J.H., Frankland, P.W., et al. CREB and memory.. Annu Rev Neurosci 21 127-148 (1998).

Heath, V.J., Gillespie, D.A.F., and Crouch, D.H. Inhibition of the terminal stages of adipocyte differentiation by cMyc. Exp Cell Res 254 91-98 (2000).

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10009846-96WELL

Transcription Factor Binding Assay Buffer (4X)

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Transcription Factor Antibody Binding Buffer (10X)

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Transcription Factor Goat Anti-Rabbit HRP Conjugate

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Transcription Factor Developing Solution

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Transcription Factor Stop Solution

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