Clone designation: RIV7 (CD4) and OKT-8 (CD8) · Host: mouse · Application(s): Flow cytometry · This antibody pair is intended for the immunochemical detection of human CD4+ and CD8+ cells by flow cytometry. Human whole blood samples may be stained directly followed by red blood cell lysis with the provided reagents. CD4 and CD8 are each expressed on a variety of white blood cells. When using the CD4/CD8 antibody pair, staining parallel samples or co-staining is recommended to identify specific cell populations. For example confirm CD4+ or CD8+ T cells as those only expressing CD3.¹ The MHC class I and II molecules of antigen presenting cells specifically interact with T cell receptor and co-receptors CD8 and CD4, respectively. CD4 and CD8 are co-expressed on T cells during development. However T cell maturation can result in distinct populations of CD4+ (helper/suppressor) or CD8+ (cytotoxic) T cells residing in the peripheral circulatory system.² Peripheral CD4+ T cell abundance is commonly employed for monitoring progression of HIV infections and other immunosuppression.^3,4 Evidence supports the existence of arachidonic acid metabolites involved in immunosuppression, immune cell maturation, differentiation, and chemotaxis.^5,6,7,8 These events have been implicated in chronic inflammation associated with the disease states such as cancer and atherosclerosis.^5,6,7,8

1 Ashmore, L.M., Shopp, G.M., and Edwards, B.S. Lymphocyte subset analysis by flow cytometry. J Immunol Methods 118 209-215 (1989).

2 Rocca, B., Spain, L.M., Puré, E., et al. Distinct roles of prostaglandin H synthase 1 and 2 in T-cell development. J Clin Invest 103(10) 1469-1477 (1999).

3 Deng, H., Liu, R., Ellmeier, W., et al. Identification of a major co-receptor for primary isolates of HIV-1. Nature 381 661-666 (1996).

4 Cosmi, L., Annunziato, F., Iwasaki, M., et al. CRTH2 is the most reliable marker for the detection of circulating human type 2 Th and type 2 T cytotoxic cells in health and disease. Eur J Immunol 30 2972-2979 (2000).

5 Bhattacharyya, S., Mandal, D., Saha, B., et al. Curcumin prevents tumor-induced T cell apoptosis through Stat-5a-mediated Bcl-2 induction. J Biol Chem 282(22) 15954-15964 (2007).

6 Tanaka, K., Ogawa, K., Sugamura, K., et al. Cutting Edge: Differential production of prostaglandin D₂ by human helper T cell subsets. J Immunol 164 2277-2280 (2000).

7 Binder, C.J., Chang, M., Shaw, P.X., et al. Innate and acquired immunity in atherogenesis. Nat Med 8(11) 1218-1226 (2002).

8 Brewer, J.A., Khor, B., Vogt, S.K., et al. T-cell glucocorticoid receptor is required to suppress COX-2-mediated lethal immune activation. Nat Med 9(10) 1318-1322 (2003).

Brewer, J.A., Khor, B., Vogt, S.K., et al. T-cell glucocorticoid receptor is required to suppress COX-2-mediated lethal immune activation. Nat Med 9(10) 1318-1322 (2003).

Binder, C.J., Chang, M., Shaw, P.X., et al. Innate and acquired immunity in atherogenesis. Nat Med 8(11) 1218-1226 (2002).

Tanaka, K., Ogawa, K., Sugamura, K., et al. Cutting Edge: Differential production of prostaglandin D₂ by human helper T cell subsets. J Immunol 164 2277-2280 (2000).

Bhattacharyya, S., Mandal, D., Saha, B., et al. Curcumin prevents tumor-induced T cell apoptosis through Stat-5a-mediated Bcl-2 induction. J Biol Chem 282(22) 15954-15964 (2007).

Cosmi, L., Annunziato, F., Iwasaki, M., et al. CRTH2 is the most reliable marker for the detection of circulating human type 2 Th and type 2 T cytotoxic cells in health and disease. Eur J Immunol 30 2972-2979 (2000).

Deng, H., Liu, R., Ellmeier, W., et al. Identification of a major co-receptor for primary isolates of HIV-1. Nature 381 661-666 (1996).

Rocca, B., Spain, L.M., Puré, E., et al. Distinct roles of prostaglandin H synthase 1 and 2 in T-cell development. J Clin Invest 103(10) 1469-1477 (1999).

Ashmore, L.M., Shopp, G.M., and Edwards, B.S. Lymphocyte subset analysis by flow cytometry. J Immunol Methods 118 209-215 (1989).

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