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Join us! · InformexUSA 2012 · New Orleans, Louisiana · February 14-17, 2012 · Booth 2514

7-AAD Cell Viability Assay Kit

Cayman Chemical Item Number 10009856

7-Amino Actinomycin D Cell Viability Kit

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Description

Evaluation of cell viability is essential in cell biology and drug discovery. Cayman’s 7-AAD Cell Viability Assay Kit employs 7-AAD as a fluorescent label for dead cells. 7-AAD is a fluorescent dye which is excluded from live cells but penetrates dead or damaged cells to label DNA. Although 7-AAD fluorescence is less intense than that of propidium iodide, it exhibits a higher wavelength emission maximum (excitation at 488 nm, emission at 650 nm) and thus has minimal spectral overlap with PE or FITC. This makes 7-AAD preferable as a viability marker when FITC and/or PE are used simultaneously to label surface or intracellular antigens. A fixative/actinomycin D solution is included in the kit for cell fixation and blocking, making subsequential immunostaining of surface/intracellular antigens possible. This kit provides a convenient tool to quantify cytotoxic effects of environmental toxins or drug candidates.
Synonyms
  • 7-Amino Actinomycin D Cell Viability Kit
Stability 1 year
Storage 4°C
Shipping Wet ice in continental US; may vary elsewhere

Background Reading

Hynes, J., Floyd, S., Soini, A.E., et al. Fluorescence-based cell viability screening assays using water-soluble oxygen probes. J Biomol Screen 8 264-272 (2003).

Coder, D.M. Assessment of cell viability. Curr Protoc Cytom 9.2.1-9.2.14 (1997).

10009856-1ea
Cell-Based Assay Buffer Tablet 4 ea
Cell-Based Assay 7-AAD Staining Stock Solution (1,000X) 1 ea
7-AAD Assay Fixative/Actinomycin D Solution 1 ea
Size Price Quantity Subtotal
1 ea $153.00 $0.00
Bulk Contact
Cart Total $0.00

This product is also available to buy in bulk quantities.

Please contact our Sales Department for a quote or to purchase.

Pricing updated 2012-02-12. Prices are subject to change without notice.

To ask for assistance with one of our products please contact a Technical Support Scientist.

Warning This product is not for human or veterinary use.

You may be eligible to receive a free sample 7-AAD Cell Viability Assay Kit under the Cayman Challenge program.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

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Cayman Chemical is a manufacturer, supplier and vendor of biochemical reagents, assay kits, antibodies, and proteins.

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Cayman Chemical Company · 1180 East Ellsworth Road · Ann Arbor, Michigan 48108 · USA

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