Antigen: recombinant adenovirus expressing full-length mouse CD36 · Clone designation: JC63.1 · Host: CD36 null mouse · Application(s): FC, functional blocking, and ICC · CD36 is a type-B scavenger receptor that is necessary for the formation of foam cells and thereby atherosclerotic lesions.^1,2,3,4 This membrane glycoprotein can internalize fatty acids which activate PPARγ and stimulate further expression of CD36.^5,6 This positive feedback loop combined with the murine CD36 knock-out studies reinforces the importance of CD36 in lipid metabolism. Additionally CD36 is needed for the phagocytosis of P. falciparum infected erythrocytes, retinal pigment epithelial cell photoreceptor fragments and post-apoptotic monocytes and neutrophils.^7,8,9,10 CD36 expression has been monitored during hematopoietic cell differentiation and may be an indicator of tumor spreading in lymphocytic leukemia.¹¹ Positive controls include adipose and heart tissue, platelets, and macrophages.

1 Febbraio, M., Abumrad, N.A., Hajjar, D.P., et al. A null mutation in murine CD36 reveals an important role in fatty acid and lipoprotein metabolism. J Biol Chem 274 19055-19062 (1999).

2 Podrez, E.A., Febbraio, M., Sheibani, N., et al. Macrophage scavenger receptor CD36 is the major receptor for LDL modified by monocyte-generated reactive nitrogen species. J Clin Invest 105(8) 1095-1108 (2000).

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4 Febbraio, M., Hajjar, D.P., and Silverstein, R.L. CD36: A class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. J Clin Invest 108(6) 785-791 (2001).

5 Han, J., Hajjar, D.P., Febbraio, M., et al. Native and modified low density lipoproteins increase the functional expression of the macrophage class B scavenger receptor, CD36. J Biol Chem 272(34) 21654-21659 (1997).

6 Tontonoz, P., Nagy, L., Alvarez, J.G.A., et al. PPARγ promotes monocyte/macrophage differentiation and uptake of oxidized LDL. Cell 93 241-252 (1998).

7 Greenwalt, D.E., Lipsky, R.H., Ockenhouse, C.F., et al. Membrane glycoprotein CD36: A review of its roles in adherence, signal transduction, and transfusion medicine. Blood 80(5) 1105-1115 (1992).

8 Finnemann, S.C., and Silverstein, R.L. Differential roles of CD36 and αvβ5 integrin in photoreceptor phagocytosis by the retinal pigment epithelium. J Exp Med 194(9) 1289-1298 (2001).

9 Navazo, M.D.P., Daviet, L., Savill, J., et al. Identification of a domain (155-183) on CD36 implicated in the phagocytosis of apoptotic neutrophils. J Biol Chem 271(26) 15381-15385 (1996).

10 Jiménez, B., Volpert, O.V., Crawford, S.E., et al. Signals leading to apoptosis-dependent inhibition of neovascularization by thrombospondin-1. Nat Med 6(1) 41-48 (2000).

11 Rutella, S., Rumi, C., Puggioni, P., et al. Expression of thrombospondin receptor (CD36) in B-cell chronic lymphocytic leukemia as an indicator of tumor cell dissemination. Haematologica 84 419-424 (1999).

Han, J., Hajjar, D.P., Febbraio, M., et al. Native and modified low density lipoproteins increase the functional expression of the macrophage class B scavenger receptor, CD36. J Biol Chem 272(34) 21654-21659 (1997).

Jiménez, B., Volpert, O.V., Crawford, S.E., et al. Signals leading to apoptosis-dependent inhibition of neovascularization by thrombospondin-1. Nat Med 6(1) 41-48 (2000).

Tontonoz, P., Nagy, L., Alvarez, J.G.A., et al. PPARγ promotes monocyte/macrophage differentiation and uptake of oxidized LDL. Cell 93 241-252 (1998).

Greenwalt, D.E., Lipsky, R.H., Ockenhouse, C.F., et al. Membrane glycoprotein CD36: A review of its roles in adherence, signal transduction, and transfusion medicine. Blood 80(5) 1105-1115 (1992).

Finnemann, S.C., and Silverstein, R.L. Differential roles of CD36 and αvβ5 integrin in photoreceptor phagocytosis by the retinal pigment epithelium. J Exp Med 194(9) 1289-1298 (2001).

Febbraio, M., Podrez, E.A., Smith, J.D., et al. Targeted disruption of the class B scavenger receptor CD36 protects against atherosclerotic lesion development in mice. J Clin Invest 105(8) 1049-1056 (2000).

Febbraio, M., Hajjar, D.P., and Silverstein, R.L. CD36: A class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism. J Clin Invest 108(6) 785-791 (2001).

Podrez, E.A., Febbraio, M., Sheibani, N., et al. Macrophage scavenger receptor CD36 is the major receptor for LDL modified by monocyte-generated reactive nitrogen species. J Clin Invest 105(8) 1095-1108 (2000).

Pearce, S.F.A., Roy, P., Nicholson, A.C., et al. Recombinant glutathione S-transferase/CD36 fusion proteins define an oxidized low density lipoprotein-binding domain. J Biol Chem 273(52) 34875-34881 (1998).

Bodart, V., Febbraio, M., Demers, A., et al. CD36 mediates the cardiovascular action of growth hormone-releasing peptides in the heart. Circ Res 90 844-849 (2002).

Navazo, M.D.P., Daviet, L., Savill, J., et al. Identification of a domain (155-183) on CD36 implicated in the phagocytosis of apoptotic neutrophils. J Biol Chem 271(26) 15381-15385 (1996).

Febbraio, M., Abumrad, N.A., Hajjar, D.P., et al. A null mutation in murine CD36 reveals an important role in fatty acid and lipoprotein metabolism. J Biol Chem 274 19055-19062 (1999).

Rutella, S., Rumi, C., Puggioni, P., et al. Expression of thrombospondin receptor (CD36) in B-cell chronic lymphocytic leukemia as an indicator of tumor cell dissemination. Haematologica 84 419-424 (1999).

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