Phosphatidylcholine Assay Kit

Cayman Chemical Item Number 10009926

PC Assay Kit

Phosphatidylcholine Assay Kit

Description

Cayman’s phosphatidylcholine (PC) assay kit provides a specific, sensitive, and convenient method for quantifying PC in plasma or serum. In this assay, PC-specific PLD is first used to hydrolyze PC to choline and phosphatidic acid. The newly formed choline is then used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue dye with an optimal absorption at 595 nm.¹

1 Hojjati, M.R., and Jiang, X. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res 47(3) 673-676 (2006).

Synonyms

PC Assay Kit

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Phillips, G.B., and Dodge, J.T. Composition of phospholipids and of phospholipid fatty acids of human plasma. J Lipid Res 8 676-681 (1967).

Jauhiainen, M., and Dolphin, P.J. Human plasma lecithin-cholesterol acyltransferase. An elucidation of the catalytic mechanism. J Biol Chem 261(15) 7032-7043 (1986).

Linsel-Nitschke, P., and Tall, A.R. HDL as a target in the treatment of atherosclerotic cardiovascular disease. Nat Rev Drug Discov 4 193-205 (2005).

Park, T., Panek, R.L., Mueller, S.B., et al. Inhibition of sphingomyelin synthesis reduces atherogenesis in apolipoprotein E-knockout mice. Circulation 110 3465-3471 (2004).

Hojjati, M.R., and Jiang, X. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res 47(3) 673-676 (2006).

Hojjati, M.R., Li, Z., Zhou, H., et al. Effect of myriocin on plasma sphingolipid metabolism and atherosclerosis in apoE-deficient mice. J Biol Chem 280(11) 10284-10289 (2005).

Matsuura, E., and Lopez, L.R. Are oxidized LDL/β₂-glycoprotein I complexes pathogenic antigens in autoimmune-mediated atherosclerosis? Clin Dev Immunol 11(2) 103-111 (2004).

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Phosphatidylcholine Standard

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