Sphingomyelin Assay Kit

Cayman Chemical Item Number 10009928

SM Assay Kit

Sphingomyelin Assay Kit

Description

Cayman’s sphingomyelin (SM) assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum. In this assay, sphingomyelinase is first used to hydrolyze SM to phosphorylcholine and ceramide. Alkaline phosphatase then generates choline from the phosphorylcholine and the newly formed choline is used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue color with an optimal absorption at 595 nm.¹

1 Hojjati, M.R., and Jiang, X. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res 47(3) 673-676 (2006).

Synonyms

SM Assay Kit

Stability

1 year

Storage

4°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Riboni, L., Viani, P., Bassi, R., et al. The role of sphingolipids in the process of signal transduction. Prog Lipid Res 36 153-195 (1997).

Testi, R. Sphingomyelin breakdown and cell fate. Trends Biochem Sci 21 468-471 (1996).

He, X., Chen, F., McGovern, M.M., et al. A fluorescence-based, high-throughput sphingomyelin assay for the analysis of Niemann-Pick disease and other disorders of sphingomyelin metabolism. Anal Biochem 306 115-123 (2002).

Schlitt, A., Blankenberg, S., Yan, D., et al. Further evaluation of plasma sphingomyelin levels as a risk factor for coronary artery disease. Nutr Metab 3(5) 1-8 (2006).

Hojjati, M.R., and Jiang, X. Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine. J Lipid Res 47(3) 673-676 (2006).

Jiang, X., Paultre, F., Pearson, T.A., et al. Plasma sphingomyelin level as a risk factor for coronary artery disease. Arterioscler Thromb Vasc Biol 20(12) 2614-2618 (2000).

Schlitt, A., Hojjati, M.R., von Gizycki, H., et al. Serum sphingomyelin levels are related to the clearance of postprandial remnant-like particles. J Lipid Res 46(2) 196-200 (2005).

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10009928-96WELL

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SM Alkaline Phosphatase

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Sphingomyelinase

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