PPARγ FL (human recombinant from Sf21 cells)

Cayman Chemical Item Number 10009987

Peroxisome Proliferator-activated Receptor γ Full Length (human recombinant from Sf21 cells)

Description

Source: human recombinant N-terminal His-tagged protein expressed in Sf21 insect cells · M_r: ~60 kDa · Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor family of ligand activated transcription factors that heterodimerizes with retinoic acid-like receptor to regulate gene expression and differentiation.¹ The PPAR family of nuclear hormone receptors consists of three subtypes encoded by separate genes: PPARα, PPARδ, and PPARγ Among them PPARγ is the most widely studied and has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer.² PPARγ is primarily expressed in adipose tissue and to a lesser extent in colon, immune system, and the retina.³ Cayman’s PPARγ full length (FL) protein is expressed and purified from Sf21 insect cells. The purity was determined using gel electrophoresis followed by coomassie staining. Ligand binding functionality was confirmed using a fluorescent-based ligand binding assay. The functionality of the DNA binding domain (DBD) was tested using Cayman’s PPARγ Transcription Factor Assay Kit (Catalog No. 10006855).

1 Kersten, S., Desvergne, B., and Wahli, W. Roles of PPARs in health and disease. Nature 405 421-424 (2000).

2 Vidal-Puig, A., Jimenez-Linan, M., Lowell, B.B., et al. Regulation of PPAR γ gene expression by nutrition and obesity in rodents. J Clin Invest 97 2553-2561 (1996).

3 Clark, R.B. The role of PPARs in inflammation and immunity. J Leukoc Biol 71 388-400 (2002).

Synonyms

Peroxisome Proliferator-activated Receptor γ Full Length (human recombinant from Sf21 cells)

Formulation

A solution in 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride, 1 mM DTT, and 20% glycerol

Purity

≥80% by SDS-PAGE

Stability

6 months

Storage

-80°C

Shipping

Dry ice in continental US; may vary elsewhere

Background Reading

Kersten, S., Desvergne, B., and Wahli, W. Roles of PPARs in health and disease. Nature 405 421-424 (2000).

Clark, R.B. The role of PPARs in inflammation and immunity. J Leukoc Biol 71 388-400 (2002).

Vidal-Puig, A., Jimenez-Linan, M., Lowell, B.B., et al. Regulation of PPAR γ gene expression by nutrition and obesity in rodents. J Clin Invest 97 2553-2561 (1996).

Size

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Subtotal

5 µg 
$72.00

$0.00

10 µg 
$137.00

$0.00

25 µg 
$324.00

$0.00

50 µg 
$576.00

$0.00

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PPARγ FL (human recombinant from Sf21 cells)