ChREBP Cell-Based Translocation Assay Kit

Cayman Chemical Item Number 10010060

Carbohydrate Response Element-Binding Protein Cell-Based Translocation Assay Kit

Description

The carbohydrate response element-binding protein (ChREBP) is a transcription factor that plays a critical role in the nutrient and hormonal regulation of genes encoding enzymes of glucose metabolism and lipogenesis.¹ Under conditions of low glucose, ChREBP is phosphorylated, which sequesters the transcription factor in the cytoplasm. Upon stimulation with glucose the protein is dephosphorylated at Ser¹⁹⁶ and then translocated to the nucleus. The activation of ChREBP has been proposed as a potential mechanism for reducing obesity and improving glucose tolerance.² Thus, identification of ChREBP activators is of great interest for drug discovery. The distinct translocation of the protein from the cytoplasm to the nucleus during activation makes it possible to study modulators of ChREBP through sub-cellular localization of the protein using conventional immunocytochemical staining with a specific antibody. Cayman’s ChREBP Cell-Based Translocation Assay Kit provides a highly specific ChREBP primary antibody together with a DyLight^™ (product of Thermo Fisher Scientific Inc.) conjugated secondary antibody in a ready to use format.

1 Uyeda, K., Yamashita, H., and Kawaguchi, T. Carbohydrate responsive element-binding protein (ChREBP): A key regulator of glucose metabolism and fat storage. Biochem Pharmacol 63 2075-2080 (2002).

2 Iizuka, K., Miller, B., and Uyeda, K. Deficiency of a carbohydrate-activated transcription factor, ChREBP, prevents obesity and improves plasma glucose control in leptin deficient (ob/ob) mice. Am J Physiol Endocrinol Metab 291 E358-E364 (2006).

Synonyms

Carbohydrate Response Element-Binding Protein Cell-Based Translocation Assay Kit

Stability

1 year

Storage

-20°C

Shipping

Wet ice in continental US; may vary elsewhere

Background Reading

Uyeda, K., Yamashita, H., and Kawaguchi, T. Carbohydrate responsive element-binding protein (ChREBP): A key regulator of glucose metabolism and fat storage. Biochem Pharmacol 63 2075-2080 (2002).

Iizuka, K., Miller, B., and Uyeda, K. Deficiency of a carbohydrate-activated transcription factor, ChREBP, prevents obesity and improves plasma glucose control in leptin deficient (ob/ob) mice. Am J Physiol Endocrinol Metab 291 E358-E364 (2006).

10010060-1EA

Cell-Based Assay Fixative

1 ea

Cell-Based Assay Blocking Solution

1 ea

DyLightTM 549-Conjugated Goat Anti-Rabbit Secondary Antibody

1 ea

ChREBP Cell-Based Assay Primary Antibody

1 ea

Cell-Based Assay Sucrose (500 mg/ml)

1 ea

Size